Anti-CDK1 (phospho T161)抗体(ab47329)

概述

  • 产品名称Anti-CDK1 (phospho T161)抗体
    参阅全部 CDK1 一抗
  • 描述
    兔多克隆抗体to CDK1 (phospho T161)
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161 (V-Y-TP-H-E).

  • 阳性对照
    • COLO cells, K562 cells, human breast carcinoma tissue.
  • 常规说明


    This antibody detects CDC2 only when phosphorylated at Threonine 161.

性能

应用

Our Abpromise guarantee covers the use of ab47329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • 组织特异性Isoform 2 is found in breast cancer tissues.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • 别名
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 (phospho T161) antibody 图像

  • ab47329 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide using Alkaline phosphatase Goat anti rabbit IgG (H+L) secondary antibody.
  • All lanes : Anti-CDK1 (phospho T161) antibody (ab47329) at 1/500 dilution

    Lane 1 : COLO cell extract preincubated with immunogenic phospho-peptide. with phosphopeptide at 1 µg/ml
    Lane 2 : COLO cell extract, untreated.
    Lane 3 : K362 cell extract

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ICC/IF image of ab47329 stained MCF7 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47329, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CDK1 (phospho T161) antibody (ab47329)参考文献

This product has been referenced in:
  • Wang J  et al. Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncol Lett 9:1266-1272 (2015). IHC ; Mouse . Read more (PubMed: 25663895) »

See 1 Publication for this product

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Thank you for your email. I have just been informed by my colleague that the blocking peptide is out of stock. Therefore they will not be able to publish the datasheet hence we are unable to fulfil your request this time. I have done my best to publish...

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Thank you for contacting us. Isotype controls are the primary antibodies that do not have specificity for any target. These can be used as negative control and are helpful in determining the background staining due to secondary antibodies. By compa...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (8% Tris Glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Andrea McCollum

Verified customer

提交于 Aug 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"