纯化说明Affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa).
功能Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
组织特异性Isoform 2 is found in breast cancer tissues.
序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
形式CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
Cdc 2 antibody
CDK 1 antibody
CELL CYCLE CONTROLLER CDC2 antibody
Cell division control protein 2 antibody
Cell division control protein 2 homolog antibody
Cell division cycle 2 G1 to S and G2 to M antibody
Cell division protein kinase 1 antibody
Cell Divsion Cycle 2 Protein antibody
Cyclin Dependent Kinase 1 antibody
Cyclin-dependent kinase 1 antibody
p34 Cdk1 antibody
p34 protein kinase antibody
Anti-CDK1 (phospho T14) antibody 图像
Western blot - Cdc2 (phospho T14) antibody (ab58509)
All lanes : Anti-CDK1 (phospho T14) antibody (ab58509) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins) Lane 2 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins), plus immunising peptide