重组Anti-CDK1 + CDK2 + CDK3 + CDK5抗体[E53] (ab32030)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CDK1 + CDK2 + CDK3 + CDK5抗体[E53]
参阅全部 CDK1+CDK2+CDK3+CDK5 一抗 -
描述
兔单克隆抗体[E53] to CDK1 + CDK2 + CDK3 + CDK5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or IHC -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Hela cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E53 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32030于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
靶标
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细胞定位
CDK1: Nucleus. CDK5: Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia. -
数据库链接
- Entrez Gene: 1017 Human
- Entrez Gene: 1018 Human
- Entrez Gene: 1020 Human
- Entrez Gene: 983 Human
- Omim: 116940 Human
- Omim: 116953 Human
- Omim: 123828 Human
- Omim: 123831 Human
see all -
形式
CDK1: CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
图片
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/10000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer 5% NFDM/TBST
The endogenous CDK is detected at higher exposure.
We are not sure the bands around 35kDa. Perhaps they are degradation products.
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/2000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Exposure time: 15 secondsBlocking/Diluting buffer 5% NFDM/TBST
34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.
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Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/5000 dilution + HeLa cell lysate
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab32030 被引用在 1 文献中.
- Ratovitski EA Phospho-?Np63a-dependent microRNAs modulate chemoresistance of squamous cell carcinoma cells to cisplatin: at the crossroads of cell life and death. FEBS Lett 587:2536-41 (2013). PubMed: 23831023