概述

  • 产品名称Anti-CDCP1抗体
    参阅全部 CDCP1 一抗
  • 描述
    山羊多克隆抗体to CDCP1
  • 特异性This antibody is expected to recognize isoform 1 (NP_073753.3) only. Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.
  • 经测试应用适用于: ICC/IF, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1.

  • 阳性对照
    • Human Colon extracts.
  • 常规说明GenBank Accession Number - NP_073753

性能

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应用

Our Abpromise guarantee covers the use of ab1377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 16823897
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).Can be blocked with Human CDCP1 peptide (ab22864).
IP Use at an assay dependent dilution. PubMed: 17785447

靶标

  • 功能May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
  • 组织特异性Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
  • 序列相似性Contains 1 CUB domain.
  • 翻译后修饰Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
  • 细胞定位Secreted and Cell membrane. Shedding may also lead to a soluble peptide.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9030022E12Rik antibody
    • AA409659 antibody
    • CD 318 antibody
    • CD_antigen=CD318 antibody
    • CD318 antibody
    • CD318 antigen antibody
    • CDCP 1 antibody
    • Cdcp1 antibody
    • CDCP1_HUMAN antibody
    • CUB domain containing protein 1 antibody
    • CUB domain-containing protein 1 antibody
    • E030027H19Rik antibody
    • FLJ13772 antibody
    • FLJ22375 antibody
    • FLJ22969 antibody
    • Membrane glycoprotein gp140 antibody
    • MGC31813 antibody
    • OTTHUMP00000164120 antibody
    • OTTHUMP00000209568 antibody
    • RGD1305578 antibody
    • SIMA 135 antibody
    • SIMA135 antibody
    • Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
    • Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
    • Transmembrane and associated with src kinases antibody
    • TRASK antibody
    see all

Anti-CDCP1 antibody 图像



  • Predicted band size : 92 kDa
  • ICC/IF image of ab1377 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1377, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab1377 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1377, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CDCP1 antibody (ab1377)参考文献

This product has been referenced in:
  • Wright HJ  et al. CDCP1 cleavage is necessary for homodimerization-induced migration of triple-negative breast cancer. Oncogene 35:4762-72 (2016). IF . Read more (PubMed: 26876198) »
  • Miura S  et al. CUB-domain containing protein 1 represses the epithelial phenotype of pancreatic cancer cells. Exp Cell Res 321:209-18 (2014). Read more (PubMed: 24384474) »

See all 11 Publications for this product

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I have been able to attain immunogen data used to create the product ab118268 for you which has been created using the full length recombinant protein. I am still awaiting further data including any additional ...

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Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that this antibody was tested by western blotting using standard tissues/cells, on this occassion human liver and brain lysates. No bands were o...

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Thank you for your enquiry. Details of the immunogens used for each of the antisera that you are interested in are as follows: I think that you may have mistyped the first antiserum as it is not directed against tubulin. CDCP1 antibody (ab1...

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I am afraid that I am unable to offer you a trial sample of this antibody for preliminary testing. The antibody is priced to reflect the fact that it is investigative grade and all the information we have is on the datasheet.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"