概述

  • 产品名称Anti-CDCP1抗体
    参阅全部 CDCP1 一抗
  • 描述
    兔多克隆抗体to CDCP1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human CDCP1.

  • 阳性对照
    • This antibody gave a positive signal in Human Skeletal Muscle tissue lysate and DU145 cell line in IF

性能

应用

Our Abpromise guarantee covers the use of ab107165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
  • 组织特异性Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
  • 序列相似性Contains 1 CUB domain.
  • 翻译后修饰Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
  • 细胞定位Secreted and Cell membrane. Shedding may also lead to a soluble peptide.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9030022E12Rik antibody
    • AA409659 antibody
    • CD 318 antibody
    • CD_antigen=CD318 antibody
    • CD318 antibody
    • CD318 antigen antibody
    • CDCP 1 antibody
    • Cdcp1 antibody
    • CDCP1_HUMAN antibody
    • CUB domain containing protein 1 antibody
    • CUB domain-containing protein 1 antibody
    • E030027H19Rik antibody
    • FLJ13772 antibody
    • FLJ22375 antibody
    • FLJ22969 antibody
    • Membrane glycoprotein gp140 antibody
    • MGC31813 antibody
    • OTTHUMP00000164120 antibody
    • OTTHUMP00000209568 antibody
    • RGD1305578 antibody
    • SIMA 135 antibody
    • SIMA135 antibody
    • Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
    • Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
    • Transmembrane and associated with src kinases antibody
    • TRASK antibody
    see all

Anti-CDCP1 antibody 图像

  • All lanes : Anti-CDCP1 antibody (ab107165) at 1 µg/ml (Milk Blocked 3%)

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330) with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 27 kDa,41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab107165 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107165, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CDCP1 antibody (ab107165)参考文献

ab107165 has not yet been referenced specifically in any publications.

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