Anti-CDCA5抗体(ab56054)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CDCA5
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to the N terminal of Human CDCA5.

性能

应用

Our Abpromise guarantee covers the use of ab56054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
  • 序列相似性
    Belongs to the sororin family.
  • 结构域
    The KEN box is required for the association with the APC/C complex.
  • 翻译后修饰
    Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
    Ubiquitinated by the APC/C complex in G1, leading to its degradation.
  • 细胞定位
    Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDCA5 antibody
    • CDCA5_HUMAN antibody
    • Cell division cycle associated protein 5 antibody
    • Cell division cycle-associated protein 5 antibody
    • MGC16386 antibody
    • p35 antibody
    • Sororin antibody
    see all

实验方案

Anti-CDCA5 antibody (ab56054)参考文献

ab56054 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
90 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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