概述

  • 产品名称Anti-CDCA3抗体
    参阅全部 CDCA3 一抗
  • 描述
    兔多克隆抗体to CDCA3
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide: ELKEGAILGT GRLLKTGGRA WEQGQDHDKE NQHFPLVES, corresponding to amino acids 230-268 of human CDCA3

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab80407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
  • 应用说明IP: Use at 2-5 µg/mg of lysate.

    Is unsuitable for WB. ab80407 has not performed satisfactorily when used for WB of CDCA3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CDCA3.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性CDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase.
    • 细胞定位Cytoplasmic. Cytosol.
    • 数据库链接
    • 别名
      • C8 antibody
      • CDCA 3 antibody
      • Cell division cycle associated 3 antibody
      • Cell division cycle associated protein 3 antibody
      • Gene rich cluster C8 antibody
      • Gene rich cluster protein C8 antibody
      • GRCC 8 antibody
      • GRCC8 antibody
      • MGC2577 antibody
      • TOME 1 antibody
      • TOME1 antibody
      • Trigger of mitotic entry 1 antibody
      • Trigger of mitotic entry protein 1 antibody
      see all

    Anti-CDCA3 antibody 图像

    • Detection of human CDCA3/TOME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80407 was used for IP at 3 µg/mg lysate (Lane 1). Lane 2 represents the control IgG. For subsequent WB, 0.1 µg/ml of ab80407 was used. Detection: Chemiluminescence with an exposure time of 3 minutes.

    Anti-CDCA3 antibody (ab80407)参考文献

    ab80407 has not yet been referenced specifically in any publications.

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