RabMAb

Anti-Cdc7 Kinase抗体[EPR4730] (ab108332)

概述

  • 产品名称Anti-Cdc7 Kinase抗体[EPR4730]
    参阅全部 Cdc7 Kinase 一抗
  • 描述
    兔单克隆抗体[EPR4730] to Cdc7 Kinase
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • Jurkat, HeLa, and PC3 cell lysates; Jurkat cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
      Contains 1 protein kinase domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cdc 7 antibody
      • CDC7 antibody
      • CDC7 cell division cycle 7 antibody
      • CDC7 cell division cycle 7 like 1 antibody
      • CDC7 L1 antibody
      • Cdc7 like 1 antibody
      • CDC7 related kinase antibody
      • CDC7-related kinase antibody
      • CDC7_HUMAN antibody
      • CDC7L 1 antibody
      • CDC7L1 antibody
      • Cell division cycle 7 homolog antibody
      • Cell division cycle 7 kinase antibody
      • Cell division cycle 7 like protein 1 antibody
      • Cell division cycle 7 related protein kinase antibody
      • Cell division cycle 7-related protein kinase antibody
      • HsCDC 7 antibody
      • HsCdc7 antibody
      • Hsk 1 antibody
      • Hsk1 antibody
      • HuCDC 7 antibody
      • huCdc7 antibody
      • MGC117361 antibody
      • MGC126237 antibody
      • MGC126238 antibody
      see all

    Anti-Cdc7 Kinase antibody [EPR4730] 图像

    • All lanes : Anti-Cdc7 Kinase antibody [EPR4730] (ab108332) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 64 kDa
    • ab108332, at 1/100 dilution, staining Cdc7 Kinase in permeabilized Jurkat cells by Flow cytometry (red). Rabbit IgG (green).

    Anti-Cdc7 Kinase antibody [EPR4730] (ab108332)参考文献

    This product has been referenced in:
    • Zhen H  et al. Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells. Evid Based Complement Alternat Med 2014:121276 (2014). WB ; Human . Read more (PubMed: 24511319) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (12)
    Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (ovary extract)
    Specification ovary extract
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    提交于 Jul 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"