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Full length protein (Human).
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab10535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 64 kDa).|
|IP||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
This image is courtesy of an Abreview submitted by Remi Buisson
Blocking Step: 1 hour at room temperature with 5% Milk in TBS.
Diluent: 5% Milk in TBS.
Image from Heffernan TP et al, J Biol Chem. 2007 Mar 30;282(13):9458-68. Epub 2007 Feb 2, Fig 7, DOI 10.1074/jbc.M611292200FLAG-Dbf4 forms a complex with endogenous Cdc7 following DNA damage. HeLa cells were transiently transfected with a FLAG-Dbf4 expression vector. 48 hours later cells were treated with 8 J/m2 UVC, 10 µm etoposide, or 2 mm HU. Cells were harvested 1 hour later and FLAG-Dbf4 immunoprecipitated with anti-FLAG-agarose (NS corresponds to immunoprecipitation with normal mouse IgG). The effect of DNA damage on Cdc7-Dbf4 complex formation was measured by Western immunoblot analysis using ab10535.
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