Anti-Cdc7 Kinase抗体(ab113956)

概述

  • 产品名称
    Anti-Cdc7 Kinase抗体
    参阅全部 Cdc7 Kinase 一抗
  • 描述
    兔多克隆抗体to Cdc7 Kinase
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Guinea pig, Cow, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 524 and 574 of Human Cdc7 Kinase (NP_003494.1).

  • 阳性对照
    • Whole cell lysate from 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab113956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdc 7 antibody
    • CDC7 antibody
    • CDC7 cell division cycle 7 antibody
    • CDC7 cell division cycle 7 like 1 antibody
    • CDC7 L1 antibody
    • Cdc7 like 1 antibody
    • CDC7 related kinase antibody
    • CDC7-related kinase antibody
    • CDC7_HUMAN antibody
    • CDC7L 1 antibody
    • CDC7L1 antibody
    • Cell division cycle 7 homolog antibody
    • Cell division cycle 7 kinase antibody
    • Cell division cycle 7 like protein 1 antibody
    • Cell division cycle 7 related protein kinase antibody
    • Cell division cycle 7-related protein kinase antibody
    • HsCDC 7 antibody
    • HsCdc7 antibody
    • Hsk 1 antibody
    • Hsk1 antibody
    • HuCDC 7 antibody
    • huCdc7 antibody
    • MGC117361 antibody
    • MGC126237 antibody
    • MGC126238 antibody
    see all

图片

  • All lanes : Anti-Cdc7 Kinase antibody (ab113956) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 64 kDa


    Exposure time : 3 minutes
  • ab113956, at 1 µg/ml, staining Cdc7 Kinase in 293T whole cell lysate immunoprecipitated using ab113956 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded). Detection: Chemiluminescence with an exposure time of 30 seconds.

文献

ab113956 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

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提交于 Aug 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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