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A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab75809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|ICC/IF||1/100 - 1/250.|
Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM /TBST. Lane 1:Cdc6 (pS54) phospho peptide, Lane 2:Cdc6 non-phospho peptide. Exposure time 3 minutes
Cell line 293T (Human embryonic kidney epithelial cell). Target AbID Ab75809 anti- Cdc6 (phospho S54)
ab150077 AlexaFluor®488 Goat anti-Rabbit secondary. Counterstain AbID Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Fixative 4% Paraformaldehyde. Permeabilisation 0.1% tritonX-100. Nuclear counter stain DAPI. Datasheet Comments Confocal image showing the expression was increased after treatment with Hydroxyurea (3mM 20hour), the signal was decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h. Target 1oAb dilution 1:100 2.4 μg/ml, Target 2ndry Ab dilution 1:1000 2 μg/ml, Counterstain Ab dilution 1:200 2.5 μg/ml
Blocking buffer and diluting buffer: 5% NFDM/TBST
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"