重组Anti-Cdc6 (phospho S106)抗体[EPR761(2)] (ab76422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR761(2)] to Cdc6 (phospho S106)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cdc6 (phospho S106)抗体[EPR761(2)]
参阅全部 Cdc6 一抗 -
描述
兔单克隆抗体[EPR761(2)] to Cdc6 (phospho S106) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab197758) -
阳性对照
- WB: Raji whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR761(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76422于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Cdc6 (phospho S106) peptide (ab197758).
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说明 |
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WB
1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Cdc6 (phospho S106) peptide (ab197758). |
靶标
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功能
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. -
序列相似性
Belongs to the CDC6/cdc18 family. -
细胞定位
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells. - Information by UniProt
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数据库链接
- Entrez Gene: 990 Human
- Omim: 602627 Human
- SwissProt: Q99741 Human
- Unigene: 405958 Human
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别名
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
图片
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All lanes : Anti-Cdc6 (phospho S106) antibody [EPR761(2)] (ab76422) at 1/1000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours then treated with 10%FBS for 24 hours whole cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours then treated with 10%FBS for 24 hours whole cell lysate, then the menbrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab76422 被引用在 2 文献中.
- Iwai K et al. Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor. Sci Adv 5:eaav3660 (2019). PubMed: 31131319
- Betts JF et al. D-amino acid oxidase is expressed in the ventral tegmental area and modulates cortical dopamine. Front Synaptic Neurosci 6:11 (2014). IF . PubMed: 24822045