概述

  • 产品名称Anti-CDC5L抗体
    参阅全部 CDC5L 一抗
  • 描述
    兔多克隆抗体to CDC5L
  • 经测试应用适用于: IP, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide derived from within residues 750 to the C-terminus of Human CDC5L.

    (Peptide available as ab30674.)

  • 阳性对照
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; A431 (Human epithelial carcinoma cell line) Whole Cell Lysate;

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
ICC/IF
IHC-P
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
    IP: Use at an assay dependent dilution (PMID 18722174).
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
    • 组织特异性Ubiquitously expressed in both fetal and adult tissues.
    • 疾病相关A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
    • 序列相似性Belongs to the CEF1 family.
      Contains 2 HTH myb-type DNA-binding domains.
    • 翻译后修饰Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
    • 细胞定位Nucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDC5 antibody
      • CDC5 cell division cycle 5-like (S. pombe) antibody
      • Cdc5 related protein antibody
      • CDC5-LIKE antibody
      • Cdc5-like protein antibody
      • Cdc5l antibody
      • CDC5L_HUMAN antibody
      • CEF1 antibody
      • Cell division cycle 5 S. pombe homolog of antibody
      • Cell division cycle 5-like protein antibody
      • dJ319D22.1 antibody
      • KIAA0432 antibody
      • PCDC5RP antibody
      • Pombe Cdc5 related protein antibody
      • Pombe Cdc5-related protein antibody
      see all

    Anti-CDC5L antibody 图像

    • All lanes : Anti-CDC5L antibody (ab31779) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : A431 whole cell lysate (ab7909)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 93 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible cleavage fragment,cross reactivity),55 kDa (possible cleavage fragment,cross reactivity).
    • ICC/IF image of ab31779 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31779, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
    • Image courtesy of Human Protein Atlas

      ab31779 staining in human stomach, showing staining of the glanular cells (in brown). Paraffin embedded lower stomach tissue was incubated with ab31779 (1:60 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab31779 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

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    Anti-CDC5L antibody (ab31779)参考文献

    This product has been referenced in:
    • van Maldegem F  et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res 43:7058-69 (2015). ICC/IF ; Mouse . Read more (PubMed: 26130721) »
    • David CJ  et al. The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex. Genes Dev 25:972-83 (2011). IP . Read more (PubMed: 21536736) »

    See all 3 Publications for this product

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