概述

  • 产品名称Anti-CDC45L抗体[EPR5758]
    参阅全部 CDC45L 一抗
  • 描述
    兔单克隆抗体[EPR5758] to CDC45L
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human CDC45L.

  • 阳性对照
    • HeLa, Jurkat, K562, and 293T cell lysates. Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol
ICC 1/50 - 1/100.
Flow Cyt 1/500 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Required for initiation of chromosomal DNA replication.
    • 组织特异性Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
    • 序列相似性Belongs to the CDC45 family.
    • 发展阶段Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDC 45 antibody
      • CDC 45L antibody
      • CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
      • CDC45 antibody
      • CDC45 cell division cycle 45 like antibody
      • CDC45 like antibody
      • CDC45 related protein antibody
      • CDC45_HUMAN antibody
      • CDC45L2 antibody
      • Cell division control protein 45 homolog antibody
      • Cell division cycle 45 like 2 antibody
      • Cell division cycle 45 like antibody
      • PORC PI 1 antibody
      • PORC-PI-1 antibody
      see all

    Anti-CDC45L antibody [EPR5758] 图像

    • All lanes : Anti-CDC45L antibody [EPR5758] (ab108350) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 66 kDa
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108350 at 1/50
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CDC45L antibody [EPR5758] (ab108350)参考文献

    This product has been referenced in:
    • Anantha RW  et al. Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination. PLoS One 8:e61368 (2013). WB . Read more (PubMed: 23585894) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab108350.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"