参阅全部 CDC45L 一抗
ELISA, WBmore details
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within internal sequence amino acids 504-553 (VTVVGIPPET DSSDRKNFFG RAFEKAAEST SSRMLHNHFD LSVIELKAED) of Human CDC45L (NP_003495).
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
- 功能Required for initiation of chromosomal DNA replication.
- 组织特异性Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
- 序列相似性Belongs to the CDC45 family.
- 发展阶段Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
- 细胞定位Cytoplasm. Nucleus.
Information by UniProt
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
Anti-CDC45L antibody 图像
Western blot - CDC45L antibody (ab82805)
Anti-CDC45L antibody (ab82805) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 66 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
Additional bands at : 25 kDa,>90 kDa. We are unsure as to the identity of these extra bands.
Anti-CDC45L antibody (ab82805)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"