概述

  • 产品名称Anti-CDC42SE2抗体
    参阅全部 CDC42SE2 一抗
  • 描述
    兔多克隆抗体to CDC42SE2
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    antigen sequence: EPTNFVHTAH VGSGDLFSGM NSVSSIQNQM QSKGGYGGGM PANVQMQLVD TKAG, corresponding to amino acids 31-84 of Human CDC42SE2.

  • 阳性对照
    • RT 4, U 251 MG, Human Liver and Human Tonsil lysates; Human small intestine tissue; A431 cells.

性能

应用

Our Abpromise guarantee covers the use of ab122492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

  • 功能Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages.
  • 组织特异性Widely expressed. Expressed at higher level in T lymphocytes. Highly expressed in CCRF-CEM T lymphocytes, Jurkat T lymphocytes, and Raji B lymphocytes compared (at protein level).
  • 序列相似性Belongs to the CDC42SE/SPEC family.
    Contains 1 CRIB domain.
  • 结构域The CRIB domain mediates interaction with CDC42.
  • 细胞定位Cytoplasm > cytoskeleton. Cell membrane. Cell projection > phagocytic cup. Recruited to the activated TCR prior actin polymerization. Localizes at the phagocytic cup of macrophages.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C42S2_HUMAN antibody
    • CDC42 small effector protein 2 antibody
    • Cdc42se2 antibody
    • FLJ21967 antibody
    • Small effector of CDC42 protein 2 antibody
    • SPEC2 antibody
    see all

Anti-CDC42SE2 antibody 图像

  • All lanes : Anti-CDC42SE2 antibody (ab122492) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab122492 at 1/50 dilution staining CDC42SE2 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
  • ab122492 at 4 µg/ml staining CDC42SE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

Anti-CDC42SE2 antibody (ab122492)参考文献

ab122492 has not yet been referenced specifically in any publications.

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