Synthetic peptide (Human) from the N-terminus of CDC42EP5.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
CDC42EP5 belongs to a family of CDC42 binding proteins that induce actin filament assembly, leading to changes in cell shape.
Cytoplasm. Cytoskeleton. Membrane; extrinsic to membrane.
- Binder of Rho GTPase 3 like antibody
- Binder of Rho GTPases 3 antibody
- BORG3 antibody
Western blot - Anti-CDC42EP5 antibody (ab75004)
All lanes :
Anti-CDC42EP5 antibody (ab75004) at 1/500 dilutionLane 1 :
Jurkat cell extractLane 2 :
Jurkat cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.Predicted band size:
15 kDaObserved band size:
22 kDa (why is the actual band size different from the predicted?
)Additional bands at:
26 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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