Anti-CDC42 Binding Protein Kinase Beta抗体(ab61328)


  • 产品名称Anti-CDC42 Binding Protein Kinase Beta抗体
    参阅全部 CDC42 Binding Protein Kinase Beta 一抗
  • 描述
    小鼠单克隆抗体to CDC42 Binding Protein Kinase Beta
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment with tag: SKMISNPTNF NHVAHMGPGD GMQVLMDLPL SAVPPSQEER PGPAPTNLAR QPPSRNKPYI SWPSSGGSEP SVTVPLRSMS DPDQDFDKEP DSDSTKHSTP , corresponding to amino acids 1580-1679 of Human CDC42 Binding Protein Kinase Beta

  • 阳性对照
    • Jurkat cell lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2b
  • 研究领域


Our Abpromise guarantee covers the use of ab61328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 194 kDa (predicted molecular weight: 194 kDa).
ELISA Use at an assay dependent dilution.


  • 功能May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
  • 组织特异性Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 CNH domain.
    Contains 1 CRIB domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC42 binding protein kinase beta antibody
    • CDC42-binding protein kinase beta antibody
    • CDC42BPB antibody
    • DMPK like beta antibody
    • DMPK-like beta antibody
    • KIAA1124 antibody
    • MRCK beta antibody
    • MRCKB_HUMAN antibody
    • Myotonic dystrophy kinase related CDC42 binding kinase beta antibody
    • Myotonic dystrophy kinase-related CDC42-binding kinase beta antibody
    • Myotonic dystrophy protein kinase like beta antibody
    • Myotonic dystrophy protein kinase-like beta antibody
    • Serine/threonine protein kinase MRCK beta antibody
    • Serine/threonine-protein kinase MRCK beta antibody
    see all

Anti-CDC42 Binding Protein Kinase Beta antibody 图像

  • Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1 µg/ml + Jurkat whole cell lysate at 50 µg

    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 194 kDa
    Observed band size : 194 kDa
  • All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution

    Lane 1 : Cytosolic fraction prepared from murine brain tissue
    Lane 2 : Cytosolic fraction prepared from murine brain tissue

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 194 kDa

    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328)参考文献

ab61328 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, cytosilic fraction)
Loading amount 10 µg
Specification brain tissue lysate, cytosilic fraction
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

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Verified customer

提交于 Apr 05 2012

Thank you for your reply.
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