Anti-CDC42 Binding Protein Kinase Beta抗体(ab61328)

概述

  • 产品名称Anti-CDC42 Binding Protein Kinase Beta抗体
    参阅全部 CDC42 Binding Protein Kinase Beta 一抗
  • 描述
    小鼠单克隆抗体to CDC42 Binding Protein Kinase Beta
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment with tag: SKMISNPTNF NHVAHMGPGD GMQVLMDLPL SAVPPSQEER PGPAPTNLAR QPPSRNKPYI SWPSSGGSEP SVTVPLRSMS DPDQDFDKEP DSDSTKHSTP , corresponding to amino acids 1580-1679 of Human CDC42 Binding Protein Kinase Beta

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2b
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab61328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 194 kDa (predicted molecular weight: 194 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
  • 组织特异性Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 CNH domain.
    Contains 1 CRIB domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC42 binding protein kinase beta antibody
    • CDC42-binding protein kinase beta antibody
    • CDC42BPB antibody
    • DMPK like beta antibody
    • DMPK-like beta antibody
    • KIAA1124 antibody
    • MRCK beta antibody
    • MRCKB_HUMAN antibody
    • Myotonic dystrophy kinase related CDC42 binding kinase beta antibody
    • Myotonic dystrophy kinase-related CDC42-binding kinase beta antibody
    • Myotonic dystrophy protein kinase like beta antibody
    • Myotonic dystrophy protein kinase-like beta antibody
    • Serine/threonine protein kinase MRCK beta antibody
    • Serine/threonine-protein kinase MRCK beta antibody
    see all

Anti-CDC42 Binding Protein Kinase Beta antibody 图像

  • Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1 µg/ml + Jurkat whole cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 194 kDa
    Observed band size : 194 kDa
  • All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution

    Lane 1 : Cytosolic fraction prepared from murine brain tissue
    Lane 2 : Cytosolic fraction prepared from murine brain tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 194 kDa


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328)参考文献

ab61328 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, cytosilic fraction)
Loading amount 10 µg
Specification brain tissue lysate, cytosilic fraction
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Verified customer

提交于 Apr 05 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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