1/100 - 1/500. Perform heat-mediated antigen retrieval with 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)
功能May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
组织特异性Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 CNH domain. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.
细胞定位Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
ab96659 staining CDC42 binding protein kinase alpha in SW480 xenograft at 1/100 dilution by Immunohistochemistry (Formalin/PFA-Fixed Paraffin-embedded sections)
Anti-CDC42 binding protein kinase alpha antibody (ab96659)参考文献
This product has been referenced in:
Zhu KC et al. Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1. Oncol Lett10:2359-2365 (2015).
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