使用敲除细胞株进行验证

Anti-CDC42抗体[M152] (ab41429)

概述

  • 产品名称
    Anti-CDC42抗体[M152]
    参阅全部 CDC42 一抗
  • 描述
    小鼠单克隆抗体[M152] to CDC42
  • 特异性
    The antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Recombinant CDC42 (Rat)

  • 阳性对照
    • human jurkat cells; mouse brain lysate
  • 常规说明
    Do not aliquot.

性能

应用

Our Abpromise guarantee covers the use of ab41429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
IHC-Fr 1/50.
WB 1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA 1/2000.

靶标

  • 功能
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • 翻译后修饰
    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
  • 别名
    • CDC42 antibody
    • CDC42_HUMAN antibody
    • CDC42Hs antibody
    • Cell division control protein 42 homolog antibody
    • Cell division cycle 42 (GTP binding protein 25kDa) antibody
    • Cell division cycle 42 antibody
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • G25K antibody
    • G25K GTP-binding protein antibody
    • Growth regulating protein antibody
    • GTP binding protein 25kDa antibody
    • Small GTP binding protein CDC42 antibody
    • TKS antibody
    see all

图片



  • Predicted band size : 21 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab41429 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab41429 was shown to specifically react with CDC42 when CDC42 knockout samples were used. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab41429 and ab181602 (loading control to GAPDH) were diluted at 1/125 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Lanes 1 & 3 : Anti-CDC42 antibody [M152] (ab41429) at 1/125 dilution
    Lanes 2 & 4 : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution

    Lane 1 : human jurkat cells
    Lane 2 : human jurkat cells
    Lane 3 : mouse brain
    Lane 4 : mouse brain

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
  • ab41429 staining CDC42 in SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence.
    SH-SY5Y cells were differentiated 6 days with 10 µM retinoic acid and fixed with 4% paraformaldehyde in PBS. After 5 minutes incubation with ice-cold methanol the cells were washed twice with PBS and blocked with 10% normal donkey serum for 30 minutes. Incubation with primary antibody was performed over night at 4°C. After three-times washing with PBS, 1 hour incubation with the labeled Cy3 secondary antibody at 37°C the cells were mounted with DABCO/Mowiol.

文献

This product has been referenced in:
  • Gaitanos TN  et al. Tiam-Rac signaling mediates trans-endocytosis of ephrin receptor EphB2 and is important for cell repulsion. J Cell Biol 214:735-52 (2016). Read more (PubMed: 27597758) »
  • Quesada-Gómez C  et al. Analysis of TcdB Proteins within the Hypervirulent Clade 2 Reveals an Impact of RhoA Glucosylation on Clostridium difficile Proinflammatory Activities. Infect Immun 84:856-65 (2016). Read more (PubMed: 26755157) »

See all 10 Publications for this product

客户评价及客户问答

Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (Egg Chamber (Ovary))
Specification
Egg Chamber (Ovary)
Username

Abcam user community

Verified customer

提交于 May 30 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole tissue lysate from ovary)
Specification
Whole tissue lysate from ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 May 30 2014

ab41429 does not detect RhoA, we have tested this directly using recombinant RhoA protein versus recombinant CDC42 protein. However, we have not tested reactivity to Rac1 and therefore, we cannot comment on the cross-reactivity with Rac1 since the epit...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo)
Specification
embryo
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
40 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Vero cells)
Loading amount
20000 cells
Specification
Vero cells
Treatment
cells expressing recombitant viral protein
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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