参阅全部 Cdc37 一抗
- 特异性ab61773 detects endogenous levels of total Cdc37 protein.
IP, WB, IHC-P, ELISAmore details
Synthesized non-phosphopeptide derived from human CDc37 around the phosphorylation site of serine 13 (E-V-SP-D-D).
- Human placenta tissue; extracts from NIH/3T3 cells.
- 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration. PubMed: 21163940
||1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
||1/50 - 1/100.
- 功能Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
- 序列相似性Belongs to the CDC37 family.
- 翻译后修饰Constitutively sumoylated by UBE2I.
Information by UniProt
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
Anti-Cdc37 antibody 图像
Western blot - Cdc37 antibody (ab61773)
All lanes : Anti-Cdc37 antibody (ab61773) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells with no peptide
Lane 2 : extracts from NIH/3T3 cells with peptide
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 34 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdc37 antibody (ab61773)
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab61773 at a dilution of 1/50. Left: untreated sample. Right: sample treated with immunising peptide.
Western blot - Anti-Cdc37 antibody (ab61773)
All lanes : Anti-Cdc37 antibody (ab61773) at 1/1000 dilution
Lane 1 : Recombinant Human Cdc37 protein (ab78881) at 0.1 µg
Lane 2 : Recombinant Human Cdc37 protein (ab78881) at 0.01 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"