概述

  • 产品名称Anti-Cdc34抗体
    参阅全部 Cdc34 一抗
  • 描述
    兔多克隆抗体to Cdc34
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 187-236 (LAEYCVKTKA PAPDEGSDLF YDDYYEDGEV EEEADSCFGD DEDDSGTEES) of Human Cdc34 (NP_004350).

  • 阳性对照
    • Human fetal thymus lysate

性能

应用

Our Abpromise guarantee covers the use of ab90300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase throught its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
  • 组织特异性Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 结构域The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.
  • 翻译后修饰Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.
    Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.
  • 细胞定位Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdc 34 antibody
    • Cdc34 antibody
    • Cell division cycle 34 antibody
    • Cell division cycle 34 homolog (S. cerevisiae) antibody
    • Cell division cycle 34 homolog antibody
    • E2 CDC34 antibody
    • UB2R1_HUMAN antibody
    • UBC 3 antibody
    • UBC3 antibody
    • UBCH3 antibody
    • UBE2 R1 antibody
    • UBE2R1 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme Cdc34 antibody
    • Ubiquitin conjugating enzyme E2 32 kDa complementing antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase R1 antibody
    • Ubiquitin-conjugating enzyme E2 R1 antibody
    • Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody
    • Ubiquitin-conjugating enzyme E2-CDC34 antibody
    • Ubiquitin-protein ligase R1 antibody
    see all

Anti-Cdc34 antibody 图像

  • Anti-Cdc34 antibody (ab90300) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa

Anti-Cdc34 antibody (ab90300)参考文献

ab90300 has not yet been referenced specifically in any publications.

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