概述

  • 产品名称Anti-CDC2L1抗体
  • 描述
    兔多克隆抗体to CDC2L1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from the N-terminus of human CDC2L1.

  • 阳性对照
    • Extracts from HepG2 and HUVEC cells.

性能

应用

Our Abpromise guarantee covers the use of ab65073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).

靶标

  • 相关性CDC2L1 is part of the Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDC2L1 is involved in cell cycle progression, cytokinesis and apoptosis. Phosphorylation at Thr-448 or Tyr-449 inactivates the enzyme, while phosphorylation at Thr-595 activates it. There are several named isoforms that arise from alternative splicing. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform may act as a negative regulator of normal cell cycle progression. The cleaved p110 isoform, p110C, binds to the serine/threonine kinase PAK1, and to RANBP9. The p58 isoform but not the p110 isoforms or p110C interacts with CCND3. The p110 isoforms are found in large molecular weight complexes containing CCNL1 and SFRS7. CDC2L1 is expressed ubiquitously and there is some evidence of isoform-specific tissue distribution. The p58 isoform is specifically induced in G2/M phase of the cell cycle. During apoptosis, induced by Fas or tumor necrosis factor, specific PITSLRE p110 isoforms are cleaved by caspases to produce a protein (p110C) that contains the C-terminal kinase domain of the PITSLRE proteins.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • CDC related protein kinase p58 antibody
    • CDC2L2 antibody
    • Cdk11 antibody
    • Cell division cycle 2 like 1 antibody
    • PITSLRE serine/threonine protein kinase CDC2L1 antibody
    • PITSLREA antibody
    • PK58 antibody
    see all

Anti-CDC2L1 antibody 图像

  • All lanes : Anti-CDC2L1 antibody (ab65073) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HUVEC cells
    Lane 3 : extracts from HUVEC cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

Anti-CDC2L1 antibody (ab65073)参考文献

ab65073 has not yet been referenced specifically in any publications.

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