概述

  • 产品名称Anti-Cdc27 (phospho T244)抗体
    参阅全部 Cdc27 一抗
  • 描述
    兔多克隆抗体to Cdc27 (phospho T244)
  • 特异性ab12281 is specific for the phosphorylated form of the Cdc27 (phospho T244). Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. The antibody does not cross-react with Cdc27 phosphorylated at other sites.
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide: C-VISPDTpVPLGT conjugated to KLH, corresponding to amino acids 240-248 of Human cdc27.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
ICC/IF
  • 应用说明ELISA: 1/3000 - 1/15000. Reactivity with non-phosphorylated human Cdc27 is minimal by ELISA.
    IF: Use at an assay dependent dilution.
    WB: 1/300 - 1/2000. Detects a band of approximately 92 kDa.
    ICC/IF: Use at an assay dependent dilution (from PubMed: 17472438).
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the APC3/CDC27 family.
      Contains 9 TPR repeats.
    • 翻译后修饰Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ANAPC3 antibody
      • Anaphase Promoting Complex 3 antibody
      • Anaphase promoting complex protein 3 antibody
      • Anaphase Promoting Complex Subunit 3 antibody
      • Anaphase-promoting complex subunit 3 antibody
      • APC 3 antibody
      • APC3 antibody
      • Cdc 27 antibody
      • Cdc27 antibody
      • CDC27 homolog antibody
      • CDC27_HUMAN antibody
      • CDC27Hs antibody
      • Cell Division Cycle 27 antibody
      • Cell division cycle protein 27 homolog antibody
      • D0S1430E antibody
      • D17S978E antibody
      • H NUC antibody
      • H-NUC antibody
      • HNUC antibody
      • Nuc2 homolog antibody
      see all

    Anti-Cdc27 (phospho T244) antibody 图像

    • All lanes : Anti-Cdc27 (phospho T244) antibody (ab12281) at 1/500 dilution

      Lane 1 : HeLa whole cell lysate from asynchronous cells
      Lane 2 : HeLa whole cell lysate from cells treated with nocodazole

      Lysates/proteins at 30 µg per lane.

      Secondary
      HRP-conjugated Goat anti-Rabbit IgG [H&L] MX at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 92 kDa (why is the actual band size different from the predicted?)

    Anti-Cdc27 (phospho T244) antibody (ab12281)参考文献

    This product has been referenced in:
    • Skoufias DA  et al. Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed. J Cell Biol 179:671-85 (2007). WB ; Human . Read more (PubMed: 18025303) »
    • Lindqvist A  et al. Cyclin B1-Cdk1 activation continues after centrosome separation to control mitotic progression. PLoS Biol 5:e123 (2007). ICC/IF ; Human . Read more (PubMed: 17472438) »

    See all 2 Publications for this product

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