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A synthetic phospho-peptide corresponding to residues surrounding Ser216 of human Cdc25C
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
Our Abpromise guarantee covers the use of ab32051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).|
Cdc25C (phospho S216) was immunoprecipitated from 10μg HEK293 (human embryonic kidney) whole cell lysate with ab32051at 1/60 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab32051 at 1/200 dilution (6 μg/ml). VeriBlot for IP (HRP) (ab131366), was used as the secondary antibody at 1/1000 dilution.
Lane 1 (Input): HEK293 (human embryonic kidney) treated with 60J/M2 UV, then 10%FBS for 60min whole cell lysate 10μg
Lane 2 (+): HEK293 (human embryonic kidney) treated with 60J/M2 UV, then 10%FBS for 60min whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32051 in HEK293 (human embryonic kidney) treated with 60J/M2 UV, then 10%FBS for 60min whole cell lysate
Exposure Time: 8 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Blocking and diluting buffer and concentration 5% NFDM/TBST.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"