Cdc25C overexpression 293T裂解液(全细胞) (ab94219)

概述

  • 产品名称Cdc25C overexpression 293T裂解液(全细胞)
  • 描述
    Cdc25C overexpression裂解液
  • 常规说明ab94219 is a 293T cell transfected lysate in which Human Cdc25C has been transiently over-expressed using a pCMV-Cdc25C plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Developmental stage: Expressed predominantly in G2 phase. Function: Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. PTM: Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Similarity: Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

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应用

Our Abpromise guarantee covers the use of ab94219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Cdc25C overexpression 293T lysate (whole cell) 图像

  • ab94219 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Cdc25C antibody (ab66235) at 1/500 dilution

    Lane 1 : Cdc25C 293T Transfected Lysate - Positive Control (ab94219)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

Cdc25C overexpression 293T lysate (whole cell) (ab94219)参考文献

ab94219 has not yet been referenced specifically in any publications.

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