Cdc25C overexpression 293T裂解物(whole cell) (ab94219)
概述
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产品名称
Cdc25C overexpression 293T裂解物(whole cell)
参阅全部 Cdc25C 细胞裂解液 -
常规说明
ab94219 is a 293T cell transfected lysate in which Human Cdc25C has been transiently over-expressed using a pCMV-Cdc25C plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Developmental stage: Expressed predominantly in G2 phase. Function: Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. PTM: Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Similarity: Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94219于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94219 at 15μg/lane on an SDS-PAGE gel.
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All lanes : Anti-Cdc25C antibody (ab66235) at 1/500 dilution
Lane 1 : Cdc25C 293T Transfected Lysate - Positive Control (ab94219)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94219 尚未被引用在任何文献中。