使用敲除细胞株进行验证

Anti-Cdc25C抗体[TC-14] (ab30313)

概述

  • 产品名称Anti-Cdc25C抗体[TC-14]
    参阅全部 Cdc25C 一抗
  • 描述
    小鼠单克隆抗体[TC-14] to Cdc25C
  • 特异性ab30313 recognises cdc25c.
  • 经测试应用适用于: ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 1-258 of human Cdc25C.

  • 表位Epitope is known to be between amino acid residues 1-150 of human cdc25c.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab30313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IP
WB
  • 应用说明IF: Use at a concentration of 2.5 µg/ml.
    IP: Use at a concentration of 1 µg/sample.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
    • 序列相似性Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • 发展阶段Expressed predominantly in G2 phase.
    • 翻译后修饰Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDC 25 antibody
      • Cdc 25C antibody
      • CDC25 antibody
      • CDC25C antibody
      • Cell division cycle 25 homolog C antibody
      • Cell division cycle 25C antibody
      • Cell division cycle 25C protein antibody
      • Dual specificity phosphatase Cdc25C antibody
      • M phase inducer phosphatase 3 antibody
      • M-phase inducer phosphatase 3 antibody
      • Mitosis inducer CDC25 antibody
      • MPIP3 antibody
      • MPIP3_HUMAN antibody
      • Phosphotyrosine phosphatase antibody
      • PPP1R60 antibody
      • protein phosphatase 1, regulatory subunit 60 antibody
      see all

    Anti-Cdc25C antibody [TC-14] 图像



    • Predicted band size : 53 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Hu bladder cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab30313 observed at 55 kDa. Red - loading control, ab181602, observed at 37 kDa.
      ab30313 was shown to recognize Cdc25C when Cdc25C knockout samples were used, along with additional cross-reactive bands. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab30313 and ab181602 (loading control to GAPDH) were diluted at 0.5 µg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    Anti-Cdc25C antibody [TC-14] (ab30313)参考文献

    ab30313 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab30313.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"