概述

  • 产品名称Anti-Cdc25C抗体[E303]
    参阅全部 Cdc25C 一抗
  • 描述
    兔单克隆抗体[E303] to Cdc25C
  • 特异性ab32050 recognises Cdc25C. It does not cross react with other MPI phosphatase family members.
  • 经测试应用适用于: WB, Flow Cyt, IPmore details
    不适用于: ICC/IF or IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C terminus of human Cdc25C.

  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
Flow Cyt 1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/30.
  • 应用说明Is unsuitable for ICC/IF or IHC.
  • 靶标

    • 功能Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
    • 序列相似性Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • 发展阶段Expressed predominantly in G2 phase.
    • 翻译后修饰Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDC 25 antibody
      • Cdc 25C antibody
      • CDC25 antibody
      • CDC25C antibody
      • Cell division cycle 25 homolog C antibody
      • Cell division cycle 25C antibody
      • Cell division cycle 25C protein antibody
      • Dual specificity phosphatase Cdc25C antibody
      • M phase inducer phosphatase 3 antibody
      • M-phase inducer phosphatase 3 antibody
      • Mitosis inducer CDC25 antibody
      • MPIP3 antibody
      • MPIP3_HUMAN antibody
      • Phosphotyrosine phosphatase antibody
      • PPP1R60 antibody
      • protein phosphatase 1, regulatory subunit 60 antibody
      see all

    Anti-Cdc25C antibody [E303] 图像



    • Predicted band size : 53 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human bladder lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32050 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32050 was shown to specifically react with Cdc25C when Cdc25C knockout samples were used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab32050 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-Cdc25C antibody [E303] (ab32050) at 1/1000 dilution + A431 cell lysate

      Predicted band size : 53 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

    Anti-Cdc25C antibody [E303] (ab32050)参考文献

    This product has been referenced in:
    • Subramaniam D  et al. Translation inhibition during cell cycle arrest and apoptosis: Mcl-1 is a novel target for RNA binding protein CUGBP2. Am J Physiol Gastrointest Liver Physiol 294:G1025-32 (2008). Read more (PubMed: 18292181) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"