概述

  • 产品名称Anti-Cdc25C抗体
    参阅全部 Cdc25C 一抗
  • 描述
    小鼠单克隆抗体to Cdc25C
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment with tag: FRSNQRKMLN LLLERDTSFT VCPDVPRTPV GKFLGDSANL SILSGGTPKC CLDLSNLSSG EITATQLTTS ADLDETGHLD SSGLQEVHLA GMNHDQHLMK CSPAQLLCST, corresponding to amino acids 21-130 of Human Cdc25C

  • 阳性对照
    • HeLa cell lysate. Human salivary gland tissue.

性能

应用

Our Abpromise guarantee covers the use of ab66235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

The detection limit for recombinant tagged Cdc25C is approximately 0.1ng/ml as a capture antibody.

IHC-P Use a concentration of 6 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • 序列相似性Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • 发展阶段Expressed predominantly in G2 phase.
  • 翻译后修饰Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC 25 antibody
    • Cdc 25C antibody
    • CDC25 antibody
    • CDC25C antibody
    • Cell division cycle 25 homolog C antibody
    • Cell division cycle 25C antibody
    • Cell division cycle 25C protein antibody
    • Dual specificity phosphatase Cdc25C antibody
    • M phase inducer phosphatase 3 antibody
    • M-phase inducer phosphatase 3 antibody
    • Mitosis inducer CDC25 antibody
    • MPIP3 antibody
    • MPIP3_HUMAN antibody
    • Phosphotyrosine phosphatase antibody
    • PPP1R60 antibody
    • protein phosphatase 1, regulatory subunit 60 antibody
    see all

Anti-Cdc25C antibody 图像



  • Predicted band size : 53 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab66235 observed at 58 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab66235 was shown to recognize Cdc25C when Cdc25C knockout samples were used, along with additional cross-reactive bands. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab66235 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • ab66235, at 6µg/ml, staining Cdc25C in human salivary gland by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • Anti-Cdc25C antibody (ab66235) at 1 µg/ml + HeLa cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

Anti-Cdc25C antibody (ab66235)参考文献

ab66235 has not yet been referenced specifically in any publications.

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