Recombinant fragment with tag: FRSNQRKMLN LLLERDTSFT VCPDVPRTPV GKFLGDSANL SILSGGTPKC CLDLSNLSSG EITATQLTTS ADLDETGHLD SSGLQEVHLA GMNHDQHLMK CSPAQLLCST, corresponding to amino acids 21-130 of Human Cdc25C
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
The detection limit for recombinant tagged Cdc25C is approximately 0.1ng/ml as a capture antibody.
Use a concentration of 6 µg/ml.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
功能Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
序列相似性Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.
发展阶段Expressed predominantly in G2 phase.
翻译后修饰Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
protein phosphatase 1, regulatory subunit 60 antibody
Anti-Cdc25C antibody 图像
Western blot - Anti-Cdc25C antibody (ab66235)
Predicted band size : 53 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Cdc25C knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lanes 1 - 3: Merged signal (red and green). Green - ab66235 observed at 58 kDa. Red - loading control, ab181602, observed at 37 kDa. ab66235 was shown to recognize Cdc25C when Cdc25C knockout samples were used, along with additional cross-reactive bands. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab66235 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.