重组Anti-Cdc25B抗体[EPR3459(2)] (ab124819)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3459(2)] to Cdc25B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cdc25B抗体[EPR3459(2)]
参阅全部 Cdc25B 一抗 -
描述
兔单克隆抗体[EPR3459(2)] to Cdc25B -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.
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阳性对照
- K562 cell lysate, U937 cell lysate and THP-1 cell lysate, mouse embryo E17 and NIH3T3 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3459(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124819于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
IP
1/10 - 1/100. |
靶标
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功能
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. -
序列相似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
翻译后修饰
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. -
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. - Information by UniProt
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数据库链接
- Entrez Gene: 994 Human
- Entrez Gene: 12531 Mouse
- Omim: 116949 Human
- SwissProt: P30305 Human
- SwissProt: P30306 Mouse
- Unigene: 153752 Human
- Unigene: 38444 Mouse
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别名
- AI604853 antibody
- Cdc 25B antibody
- Cdc25b antibody
see all
图片
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All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 0.486 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) cell lysates
Lane 2 : Mouse embryo E17 lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/diluting Buffer and concentration: 5% NFDM /TBST -
All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab124819 被引用在 5 文献中.
- Du Y et al. SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression. J Exp Clin Cancer Res 41:185 (2022). PubMed: 35624501
- Zhao J et al. lncRNA LIFR-AS1 inhibits gastric carcinoma cell proliferation, migration and invasion by sponging miR-4698. Mol Med Rep 23:N/A (2021). PubMed: 33355363
- Ding L et al. Cdc25B is transcriptionally inhibited by IER5 through the NF-YB transcription factor in irradiation-treated HeLa cells. Toxicol Res (Camb) 10:875-884 (2021). PubMed: 34484679
- Wang K et al. Corosolic acid induces cell cycle arrest and cell apoptosis in human retinoblastoma Y-79 cells via disruption of MELK-FoxM1 signaling. Oncol Rep 39:2777-2786 (2018). PubMed: 29620242
- Zhong Y et al. KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. Oncogene 36:6177-6189 (2017). PubMed: 28869606