Anti-Cdc25A (phospho S178)抗体(ab79252)

概述

  • 产品名称Anti-Cdc25A (phospho S178)抗体
    参阅全部 Cdc25A 一抗
  • 描述
    兔多克隆抗体to Cdc25A (phospho S178)
  • 特异性Detects Cdc25A only when phosphorylated at serine 178
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human Cdc25A around the phosphorylation site of serine 178 (Q-N-SP-A-P)

  • 阳性对照
    • WB: extracts from UV-treated A549 cells; IHC-P: Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab79252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
  • 序列相似性Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • 结构域The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
  • 翻译后修饰Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdc 25a antibody
    • CDC25A antibody
    • CDC25A2 antibody
    • CDC25A2 CAG isoform antibody
    • Cell division cycle 25 homolog A (S. pombe) antibody
    • Cell division cycle 25A antibody
    • Cell division cycle 25A isoform a antibody
    • Cell division cycle 25A isoform b antibody
    • D9Ertd393e antibody
    • Dual specificity phosphatase Cdc25A antibody
    • M phase inducer phosphatase 1 antibody
    • M-phase inducer phosphatase 1 antibody
    • MGC115549 antibody
    • MPIP1_HUMAN antibody
    see all

Anti-Cdc25A (phospho S178) antibody 图像

  • Left panel: immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using 1/50 ab79252. Right panel: same, after pre-incubation with the immunizing peptide.
  • All lanes : Anti-Cdc25A (phospho S178) antibody (ab79252) at 1/500 dilution

    Lane 1 : Extracts from A549 cell treated with UV (15mins)
    Lane 2 : Extracts from A549 cell treated with UV (15mins) with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 110 kDa,117 kDa. We are unsure as to the identity of these extra bands.

Anti-Cdc25A (phospho S178) antibody (ab79252)参考文献

ab79252 has not yet been referenced specifically in any publications.

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