Anti-Cdc25A抗体[DCS-120 + DCS-121] (ab2357)


  • 产品名称Anti-Cdc25A抗体[DCS-120 + DCS-121]
    参阅全部 Cdc25A 一抗
  • 描述
    小鼠单克隆抗体[DCS-120 + DCS-121] to Cdc25A
  • 经测试应用适用于: WB, IP, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • Tonsil.



Our Abpromise guarantee covers the use of ab2357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/50 - 1/100. ABC method.


  • 功能Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
  • 序列相似性Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • 结构域The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
  • 翻译后修饰Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdc 25a antibody
    • CDC25A antibody
    • CDC25A2 antibody
    • CDC25A2 CAG isoform antibody
    • Cell division cycle 25 homolog A (S. pombe) antibody
    • Cell division cycle 25A antibody
    • Cell division cycle 25A isoform a antibody
    • Cell division cycle 25A isoform b antibody
    • D9Ertd393e antibody
    • Dual specificity phosphatase Cdc25A antibody
    • M phase inducer phosphatase 1 antibody
    • M-phase inducer phosphatase 1 antibody
    • MGC115549 antibody
    • MPIP1_HUMAN antibody
    see all

Anti-Cdc25A antibody [DCS-120 + DCS-121] 图像

  • ab2357 staining human tonsil by IHC-P.

Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)参考文献

This product has been referenced in:
  • Sims JK & Wade PA Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin. Mol Biol Cell 22:3094-102 (2011). Read more (PubMed: 21737684) »
  • Liberal V  et al. Cyclin-dependent kinase subunit (Cks) 1 or Cks2 overexpression overrides the DNA damage response barrier triggered by activated oncoproteins. Proc Natl Acad Sci U S A : (2011). WB ; Human . Read more (PubMed: 21697511) »

See all 4 Publications for this product

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (BT474 Xenografts)
Specification BT474 Xenografts
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Normal Goat Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C

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Verified customer

提交于 Mar 17 2010

The concentration is 100 ug/ml. If you have any additional questions, please contact us again.

Thank you for your enquiry and providing more details of your protocol. This antibody has been optimized for paraffin tissue sections. We would suggest using at 1:25, 1:50 for 30 min. Pretreatment with Citrate buffer. Use tonsil as positive control alo...

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