概述

  • 产品名称Anti-Cdc25A抗体
    参阅全部 Cdc25A 一抗
  • 描述
    兔多克隆抗体to Cdc25A
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).

性能

应用

Our Abpromise guarantee covers the use of ab989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 1 - 4 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).

靶标

  • 功能Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
  • 序列相似性Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • 结构域The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
  • 翻译后修饰Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdc 25a antibody
    • CDC25A antibody
    • CDC25A2 antibody
    • CDC25A2 CAG isoform antibody
    • Cell division cycle 25 homolog A (S. pombe) antibody
    • Cell division cycle 25A antibody
    • Cell division cycle 25A isoform a antibody
    • Cell division cycle 25A isoform b antibody
    • D9Ertd393e antibody
    • Dual specificity phosphatase Cdc25A antibody
    • M phase inducer phosphatase 1 antibody
    • M-phase inducer phosphatase 1 antibody
    • MGC115549 antibody
    • MPIP1_HUMAN antibody
    see all

Anti-Cdc25A antibody 图像



  • Predicted band size : 59 kDa

    ab989 (Rabbit polyclonal to exon 7 of human Cdc25a) and ab991 (Rabbit polyclonal to exon 9 of human Cdc25a) both at a dilution of 1:2000, staining 500µg HeLa whole cell extract by Immunoprecipitation / Western Blot (ECL)

Anti-Cdc25A antibody (ab989)参考文献

This product has been referenced in:
  • Liang J  et al. PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nat Commun 7:12431 (2016). WB ; Human . Read more (PubMed: 27485204) »
  • Brunetto E  et al. CDC25A protein stability represents a previously unrecognized target of HER2 signaling in human breast cancer: implication for a potential clinical relevance in trastuzumab treatment. Neoplasia 15:579-90 (2013). Read more (PubMed: 23730206) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT29 tumour cells)
Loading amount 50 µg
Specification HT29 tumour cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Paul Eve

Verified customer

提交于 Jan 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"