概述

  • 产品名称Anti-Cdc20抗体
    参阅全部 Cdc20 一抗
  • 描述
    兔多克隆抗体to Cdc20
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Cdc20.

    (Peptide available as ab28138.)

  • 阳性对照
    • This antibody gave a positive result in the following whole cell lysates: Raji (Human Burkitt's lymphoma cell line) THP1 (Human acute monocytic leukemia cell line) HEK 293 (Human embryonic kidney cell line) HL60 (Human promyelocytic leukemia cell line)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab26483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • 发展阶段Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
  • 翻译后修饰Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
    Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA276H19.3 antibody
    • Cdc 20 antibody
    • CDC20 antibody
    • CDC20 cell division cycle 20 homolog antibody
    • CDC20_HUMAN antibody
    • CDC20A antibody
    • Cell division cycle 20 antibody
    • Cell division cycle 20 homolog (S. cerevisiae) antibody
    • Cell division cycle 20 homolog antibody
    • Cell division cycle protein 20 homolog antibody
    • fizzy antibody
    • MGC102824 antibody
    • p55CDC antibody
    see all

Anti-Cdc20 antibody 图像

  • All lanes : Anti-Cdc20 antibody (ab26483) at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908)
    Lane 2 : THP1 whole cell lysate (ab7913)
    Lane 3 : HEK293 whole cell lysate (ab7902)
    Lane 4 : HL60 whole cell lysate (ab7914)

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
  • ICC/IF image of ab26483 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab26483, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-Cdc20 antibody (ab26483)参考文献

This product has been referenced in:
  • Lu H  et al. The E3 ligase APC/C-Cdh1 regulates MEF2A-dependent transcription by targeting SUMO-specific protease 2 for ubiquitination and degradation. Cell Cycle 13:3892-902 (2014). WB . Read more (PubMed: 25483061) »
  • Chen M  et al. The anaphase-promoting complex or cyclosome supports cell survival in response to endoplasmic reticulum stress. PLoS One 7:e35520 (2012). WB ; Human . Read more (PubMed: 22539978) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 10 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
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提交于 Mar 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"