概述

  • 产品名称Anti-CDAN1抗体
    参阅全部 CDAN1 一抗
  • 描述
    兔多克隆抗体to CDAN1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CDAN1.

    (Peptide available as ab28453.)

  • 阳性对照
    • Hela B Cell Lysate, Human Ovary Tissue Lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Might be involved in nuclear membrane integrity.
  • 组织特异性Ubiquitously expressed. Isoform 3 is not found in erythroid cells.
  • 疾病相关Defects in CDAN1 are the cause of congenital dyserythropoietic anemia type 1 (CDA1) [MIM:224120]. An autosomal recessive blood disorder characterized by morphological abnormalities of erythroblasts, ineffective erythropoiesis, macrocytic anemia and secondary hemochromatosis. It is occasionally associated with bone abnormalities, especially of the hands and feet (acrodysostosis), nail hypoplasia, and scoliosis. Ultrastructural features include internuclear chromatin bridges connecting some nearly completely separated erythroblasts and an abnormal appearance (spongy or Swiss-cheese appearance) of the heterochromatin in a high proportion of the erythroblasts.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDA1 antibody
    • CDAI antibody
    • CDAN1 antibody
    • CDAN1_HUMAN antibody
    • Codanin antibody
    • Codanin-1 antibody
    • Codanin1 antibody
    • PRO1295 antibody
    see all

Anti-CDAN1 antibody 图像

  • All lanes : Anti-CDAN1 antibody (ab31236) at 0.5 µg/ml

    Lane 1 : Hela B Cell Lysate at 30 µg
    Lane 2 : Hela B Cell Lysate at 60 µg


    Predicted band size : 134 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 170 kDa (possible glycosylated form,non-specific binding).

    This image is courtesy of the laboratory of Genevieve Almouzni

    CDAN1 antibody ab31236 detected a band of 130 kDa doublet in Hela B lysate that is likely to correspond to CDAN1. Staining of tubulin in the same lanes is shown as a loading control. The identity of the band at 170 kDa is not known.
  • ICC/IF image of ab31236 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31236, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Anti-CDAN1 antibody (ab31236) at 1 µg/ml + Human ovary tissue lysate - total protein (ab30222) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 134 kDa
    Additional bands at : 145 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

Anti-CDAN1 antibody (ab31236)参考文献

ab31236 has not yet been referenced specifically in any publications.

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