Anti-CDA抗体- C-terminal (ab82346)

概述

  • 产品名称Anti-CDA抗体- C-terminal
    参阅全部 CDA 一抗
  • 描述
    兔多克隆抗体to CDA - C-terminal
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human CDA aa 118-146 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 978

  • 阳性对照
    • WB: HeLa cell lysate and mouse kidney tissue lysate. ICC/IF: HepG2 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明ab82346 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

靶标

  • 相关性CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.
  • 数据库链接
  • 别名
    • CDD antibody
    • Cytidine aminohydrolase antibody
    • Cytidine deaminase antibody
    • EC 3.5.4.5 antibody

Anti-CDA antibody - C-terminal 图像

  • All lanes : Anti-CDA antibody - C-terminal (ab82346) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 16 kDa
  • ab82346 staining CDA in murine pancreatic adenocarcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 5% serum for 1 hour at 25°C and then incubated with ab82346 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an undiluted HRP conjugated horse anti-rabbit polyclonal.

    See Abreview

  • ab82346 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82346 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CDA antibody - C-terminal (ab82346)参考文献

This product has been referenced in:
  • Damhofer H  et al. Assessment of the stromal contribution to Sonic Hedgehog-dependent pancreatic adenocarcinoma. Mol Oncol N/A:N/A (2013). Read more (PubMed: 23998958) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (lung cancer cells)
Loading amount 50000 cells
Specification lung cancer cells
Treatment with 10 nM DMSO or paclitaxel for 16 hours
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 27 2012

rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (murine pancreatic adenocarcinoma)
Specification murine pancreatic adenocarcinoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

提交于 Jan 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (multiple tissues)
Loading amount 40 µg
Specification multiple tissues
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kris Frese

Verified customer

提交于 Jan 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"