Anti-CD99抗体[DN16] (ab23617)
Key features and details
- Mouse monoclonal [DN16] to CD99
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-CD99抗体[DN16]
参阅全部 CD99 一抗 -
描述
小鼠单克隆抗体[DN16] to CD99 -
宿主
Mouse -
特异性
ab23617 recognises the human CD99 cell surface antigen, a 32kD glycoprotein expressed by all leucocytes. CD99 expression tends to be highest on immature cells. -
经测试应用
适用于: IHC-Fr, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
The details of the immunogen for this antibody are not available.
-
表位
Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence 'LPDNENKK' located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
DN16 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23617于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. -
序列相似性
Belongs to the CD99 family. -
翻译后修饰
Extensively O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 4267 Human
- Omim: 313470 Human
- SwissProt: P14209 Human
- Unigene: 653349 Human
-
别名
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
see all
实验方案
数据表及文件
-
Datasheet download
文献 (4)
ab23617 被引用在 4 文献中.
- Cardoso LC et al. CD99 Expression in Glioblastoma Molecular Subtypes and Role in Migration and Invasion. Int J Mol Sci 20:N/A (2019). PubMed: 30845661
- O'Neill AF et al. Targeted imaging of Ewing sarcoma in preclinical models using a 64Cu-labeled anti-CD99 antibody. Clin Cancer Res 20:678-87 (2014). PubMed: 24218512
- Rabquer BJ et al. The proadhesive phenotype of systemic sclerosis skin promotes myeloid cell adhesion via ICAM-1 and VCAM-1. Rheumatology (Oxford) 48:734-40 (2009). ELISA, IHC-Fr ; Human . PubMed: 19439502
- Kim SH et al. Viral latent membrane protein 1 (LMP-1)-induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype. Blood 95:294-300 (2000). PubMed: 10607715