概述

  • 产品名称
    Anti-CD99抗体[12E7]
    参阅全部 CD99 一抗
  • 描述
    小鼠单克隆抗体[12E7] to CD99
  • 宿主
    Mouse
  • 特异性
    This antibody has been shown to give appropriate reactivity on a wide range of formalin-fixed, paraffin-embedded human tissue sections.
  • 经测试应用
    适用于: IP, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation: Acute lymphocytic leukemia T-cells.

性能

应用

Our Abpromise guarantee covers the use of ab8855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能
    Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • 序列相似性
    Belongs to the CD99 family.
  • 翻译后修饰
    Extensively O-glycosylated.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 12E7 antibody
    • Antigen identified by monoclonal 12E7, Y homolog antibody
    • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7 antibody
    • Cell surface antigen HBA 71 antibody
    • Cell surface antigen O13 antibody
    • E2 antigen antibody
    • HBA71 antibody
    • MIC 2X antibody
    • MIC 2Y antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Protein MIC2 antibody
    • Surface antigen MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

图片

  • ab8855 staining CD99 in the Human Ewing Sarcoma Celline CHP cell line by Flow Cytometry. The sample was incubated with the primary antibody (1/60 in PBS) for 30 minutes at 4°C. A FITC-conjugated Goat anti-mouse IgG (1/500) was used as the secondary antibody.
    Gating Strategy: Live Cells.

    See Abreview

文献

This product has been referenced in:
  • Hong SH  et al. Pharmacokinetic modeling optimizes inhibition of the 'undruggable' EWS-FLI1 transcription factor in Ewing Sarcoma. Oncotarget 5:338-50 (2014). Human . Read more (PubMed: 24481407) »

See 1 Publication for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Ewing Sarcoma Celline CHP)
Specification
Ewing Sarcoma Celline CHP
Fixation
No Fixation
Permeabilization
No
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提交于 Aug 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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