重组
RabMAb

Anti-CD97抗体[EPR4427] (ab108368)

概述

  • 产品名称
    Anti-CD97抗体[EPR4427]
    参阅全部 CD97 一抗
  • 描述
    兔单克隆抗体[EPR4427] to CD97
  • 经测试应用
    适用于: Flow Cyt, WB, IP, IHC-P, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the extracellular domain of Human CD97.

  • 阳性对照
    • Human tonsil, Jurkat, U937, and fetal thymus lysates; Human spleen, colon adenocarcinoma and tonsil tissues. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDA-MB-231
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/250 - 1/500.
ICC/IF 1/200.

靶标

  • 功能
    Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
  • 组织特异性
    Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
  • 序列相似性
    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 5 EGF-like domains.
    Contains 1 GPS domain.
  • 结构域
    The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
    Binding to chondroitin sulfate is mediated by the fourth EGF domain.
  • 翻译后修饰
    Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
  • 细胞定位
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADGRE5 antibody
    • Adhesion G protein-coupled receptor E5 antibody
    • CD 97 antibody
    • CD_antigen=CD97 antibody
    • CD97 antibody
    • CD97 antigen antibody
    • CD97 antigen subunit beta antibody
    • CD97 molecule antibody
    • CD97_HUMAN antibody
    • Leukocyte antigen CD97 antibody
    • Seven span transmembrane protein antibody
    • Seven transmembrane helix receptor antibody
    • Seven transmembrane heterodimeric receptor associated with inflammation antibody
    • TM7LN 1 antibody
    • TM7LN1 antibody
    see all

图片

  • ICC/IF image of ab108368 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108368 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab108368 at 1/250 dilution staining CD97 in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

  • Flow Cytometry analysis of MDA-MB-231 (human mammary gland epithelial adenocarcinoma) cells labeling CD97 with purified ab108368 at 1/250 dilution (10ug/ml) (red). A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • All lanes : Anti-CD97 antibody [EPR4427] (ab108368) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : Human fetal thymus lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 92 kDa
  • ab108368 at 1/250 dilution staining CD97 in human spleen by Immunohistochemistry, Paraffin-embedded tissue.

  • ab108368 at 1/250 dilution staining CD97 in human colon adenocarcinoma tissue by Immunohistochemistry, Paraffin-embedded tissue.

文献

This product has been referenced in:
  • Zhao W  et al. Lentivirus-mediated overexpression of CD97/ADGRE5 reverses dysregulated high glucose-induced endothelial cell migration. Mol Med Rep 15:3048-3054 (2017). WB ; Human . Read more (PubMed: 28358422) »
  • Safaee M  et al. Proportional upregulation of CD97 isoforms in glioblastoma and glioblastoma-derived brain tumor initiating cells. PLoS One 10:e0111532 (2015). WB, ICC . Read more (PubMed: 25714433) »

See all 3 Publications for this product

客户评价及客户问答



J’ai contacté le laboratoire qui m’a confirmé que les IgG de lapins n’ont pas de sous-classes comme les IgG de souris et d'humains. L’isotype d’ab108368 est donc simplement IgG.

Thank you for contacting us.

I have spoken to my colleague who suggested ab108368 (http://www.abcam.com/ab108368) to you. This antibody is a rabbit monoclonal which has been tested in immunocytochemistry, immunohistochemistry (paraffin), im...

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Thank you for your response and for your continued patronage of Abcam. I am glad that you enjoyed the brownies!

When ordering, it is most beneficial to use our website or to call our customer service representatives as they are much more kno...

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I has been my pleasure to help with this. I would like to thank you for your help as well and for your cooperation. The details that you have provided will enable us to closely monitor the quality of our products.

I am sorry this product d...

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Thank you for sending me this further information. It has helped tremendously in understanding the issues that you have been facing.

I agree with your assessment that these bands are either non-specific or isoforms 4 and 5. As we cannot be s...

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I am sorry about the issues you have been having. Thank you for bringing this to our attention. As this product should not be recognizing the other forms of EMR2 it appears that you may be experiencing non specific background issues. I may be able to h...

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I have had to opportunity to speak with the lab further regarding this. The immunogen sequence for this product is:XXX which spans aaXXX-XXX (SwissProt: Q9UHX3Human). This sequence is within the excellular region and is not in isoforms 4 and 5. Are ...

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Thank you for contacting us.

I have had the opportunity to speak with the labs in regard to your questions about these product.

The immunogen sequence for each product is proprietary but the labs have run alignments and checked ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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