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A synthetic peptide corresponding to residues in the extracellular domain of Human CD97.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab108368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Antigen retrieval is recommended.|
|ICC||1/250 - 1/500.|
ICC/IF image of ab108368 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108368 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab108368 at 1/250 dilution staining CD97 in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Flow Cytometry analysis of MDA-MB-231 (human mammary gland epithelial adenocarcinoma) cells labeling CD97 with purified ab108368 at 1/250 dilution (10ug/ml) (red). A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
ab108368 at 1/250 dilution staining CD97 in human spleen by Immunohistochemistry, Paraffin-embedded tissue.
ab108368 at 1/250 dilution staining CD97 in human colon adenocarcinoma tissue by Immunohistochemistry, Paraffin-embedded tissue.
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