Anti-CD90 / Thy1抗体[IBL-6/23] (ab3105)

概述

  • 产品名称Anti-CD90 / Thy1抗体[IBL-6/23]
    参阅全部 CD90 / Thy1 一抗
  • 描述
    大鼠单克隆抗体[IBL-6/23] to CD90 / Thy1
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cyt, IHC-Fr, IP, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Mouse spleen cells.

性能

应用

Our Abpromise guarantee covers the use of ab3105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use a concentration of 1 - 10 µg/ml. Fix with acetone.
IP Use a concentration of 1 - 10 µg/ml.
IHC-FoFr Use a concentration of 1 - 10 µg/ml.

靶标

  • 功能May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Anti-CD90 / Thy1 antibody [IBL-6/23] 图像

  • ab3105 used in Flow Cytometry.
    ab3105 used at 5µg/ml to stain murine thymus cells. The secondary used was an Alexa Fluor 488 conjugated goat anti-rat IgG monoclonal, used at a 1/1000 dilution.

    See Abreview

  • ab3105 staining CD90 / Thy1 in murine bone marrow cells (smeared directly onto glass slide) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, blocked with 1% serum for 1 hour at 37°C and then incubated with ab3105 at a 1/300 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rat polyclonal used at a 1/400 dilution. Dapi stains nuclei in blue.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffiin-embedded sections) analysis of mouse spleen tissue labelling CD90 / Thy1 with ab3105 at 1/100. Antigen retrieval was heat-mediated with citrate buffer pH 6.0.

Anti-CD90 / Thy1 antibody [IBL-6/23] (ab3105)参考文献

This product has been referenced in:
  • Feng W  et al. Isolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells. Asian-Australas J Anim Sci 29:1407-15 (2016). IF . Read more (PubMed: 26954139) »
  • Adams AE  et al. Mitochondrial uncoupling protein 1 expression in thymocytes. Biochim Biophys Acta 1777:772-6 (2008). Read more (PubMed: 18471433) »

See all 2 Publications for this product

Product Wall

I can confirm that ab3105 was tested on formalin-fixed paraffin-embedded tissue sections from human reactive tonsil but we didn't detect signal with this antibody.

Application Immunohistochemistry (Frozen sections)
Blocking step NOVOCASTRA protein block as blocking agent as blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Sample Mouse Tissue sections (Mouse skeletal muscle)
Specification Mouse skeletal muscle
Permeabilization Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Feb 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bone marrow cells smear)
Specification Bone marrow cells smear
Fixative Paraformaldehyde
Permeabilization Yes - saponin 0.5%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
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Ms. Sophie Nadaud

Verified customer

提交于 Feb 23 2012

Thank you for contacting us. Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Thymus)
Specification Thymus
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Jul 14 2011

Thank you for your enquiry, we have not tested the antibody ab3105 in immunocytochemistry but the antibody is very likely to work as it works very well in perfusion fixed tissue and frozen post fixed tissue. I would definitely recommend fixing mous...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"