Anti-CD90 / Thy1抗体[EPR3132] (ab92574)


  • 产品名称Anti-CD90 / Thy1抗体[EPR3132]
    参阅全部 CD90 / Thy1 一抗
  • 描述
    兔单克隆抗体[EPR3132] to CD90 / Thy1
  • 特异性This antibody reacts with mouse samples in WB, but does not work on mouse samples in other applications. Rat cross-reactivity has been tested by western blot only.
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminal of Human CD90/ Thy1 (UniProt ID: P04216).

  • 阳性对照
    • Fetal brain, fetal kidney lysates, Human breast carcinoma, Human glioma and Human brain tissue
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab92574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 18 kDa.

can be blocked with ab134486

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    Anti-CD90 / Thy1 antibody [EPR3132] 图像

    • All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
    • ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab92574 showing positive staining in Normal tonsil vessels tissue.

    • ab92574 showing positive staining in Skeletal muscle vessels tissue.

    • ab92574 showing positive staining in Cervical carcinoma tissue.

    • ab92574 showing positive staining in Lung adenocarcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)参考文献

    This product has been referenced in:
    • Peng YC  et al. Combination of 5-fluorouracil and 2-morphilino-8-phenyl-4H-chromen-4-one may inhibit liver cancer stem cell activity. Tumour Biol N/A:N/A (2016). Human . Read more (PubMed: 26886287) »
    • Wang X  et al. Influence of the intensity and loading time of direct current electric field on the directional migration of rat bone marrow mesenchymal stem cells. Front Med N/A:N/A (2016). Read more (PubMed: 27324024) »

    See all 10 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Bone marrow biopsy)
    Antigen retrieval step None
    Permeabilization No
    Specification Bone marrow biopsy
    Blocking step (agent) for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative 10% NBF

    Premier Laboratory

    Verified customer

    提交于 Mar 29 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step None
    Sample Human Tissue sections (skin)
    Specification skin
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Jun 13 2014

    Thank you for contacting us.

    The concentration for ab32570 lot GR7483-3 is XXX

    Conc. for ab92574 lot GR37919-4 is XXXX

    Please note these are rabbit monoclonals antibodies. They are very specific to their targets so the workin...

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    Thank you for contacting us.
    As discussed over the phone, could you please send me the lot numbers?
    Thank you.

    Thank you for contacting us.

    The concentration of the current lot we have in stock (lot GR37919)is 0.0705 mg/mL.

    The blocking peptide has been added to the catalog as ab134486 (http://www.abcam.com/cd90--thy1-peptide-ab134486.html...

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    Thank you for contacting us.

    I have contacted the lab for the lot concentration as well as for the blocking peptide, and will let you know when I hear back.

    The link to the Human Protein Atlas for human Thy1 is as follows:

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    Thank you for your patience. The lab has confirmed that ab92574 was tested for Flow Cytometry and gave negative results. We do not have any antibodies for CD90 that are known to give good signal in rat but no signal in mouse, and unfortunately I do not...

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    Thank you for submitting the completed questionnaire and for including the ICC images.

    It seems like the few cells that are stained is just the result of non specific background staining.

    A couple additional questions:

    1. ...

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    I apologize for the confusion of the datasheet for ab92574. After checking our notes about the non-reactivity with mouse, I found that this antibody does work on mouse samples in WB, but has not worked in mouse samples in other applications. The rat cr...

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    Thank you for your email. The overall sequence homology for the full length proteins are: human-cow: 74% mouse-cow: 69% rat-cow: 70% The majority of our CD90 antibodies were raised against full length proteins or cell lines and therefore the...

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