Anti-CD90 / Thy1抗体[AF-9] (ab23894)

概述

  • 产品名称
    Anti-CD90 / Thy1抗体[AF-9]
    参阅全部 CD90 / Thy1 一抗
  • 描述
    小鼠单克隆抗体[AF-9] to CD90 / Thy1
  • 经测试应用
    适用于: IHC-Fr, Flow Cyt, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human) T lymphoma cells.

性能

应用

Our Abpromise guarantee covers the use of ab23894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • 序列相似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

图片

  • Overlay histogram showing Jurkat cells stained with ab23894 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23894, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab23894 staining CD90/Thy1 in Human myometrial tissue sections by IHC-Fr (frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50) for 18 hours. An undiluted Biotin-conjugated donkey anti-mouse IgG polyclonal was used as secondary antibody.

    See Abreview

  • ab23894 staining CD90 / Thy1 in Limbal biopsies from Human corneas by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and blocked with 10% goat serum in PBS for 90 minutes at room temperature. Samples were incubated with primary antibody overnight at 4°C. An Alexa Fluor® 594-conjugated Goat anti-mouse polyclonal (1/250) was used as the secondary antibody.

    CD90 expression is markedly increased by limbal stromal cells underlying the limbal crypts (E) compared to the non-crypt rich limbus (C). Central corneal sections were used as a negative control (A). Sections were counterstainded with DAPI (blue). Scale bars: 50um.

文献

This product has been referenced in:
  • Zhang GP  et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). Flow Cyt ; Human . Read more (PubMed: 27860183) »
  • Dziasko MA  et al. Localisation of epithelial cells capable of holoclone formation in vitro and direct interaction with stromal cells in the native human limbal crypt. PLoS One 9:e94283 (2014). IHC-Fr ; Human . Read more (PubMed: 24714106) »

See all 5 Publications for this product

客户评价及客户问答

Thank you for contacting Abcam.
Earlier today we discussed the Human Mesenchymal Stromal Cell Marker panel ab93758. Based on the information on the datasheet, I compiled the following information for you:
1. Positive selection (CD105, CD29, CD4...

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Thank you for your enquiry and interest in our products.

I can confirm that this panel contains 5 primary antibodies which recognize human mesenchymal stromal cells: ab6124, ab10559, ab23894, ab44967, ab52971. The datasheets can be found at t...

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Thank you for contacting Abcam earlier today.
I am sorry that you were not getting any staining using ab23894 in IHC-Fr. As we discussed, I have processed a refund, and your credit note number is ********.
Please let me know if there is anyth...

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Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not be...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (myometrium)
Specification
myometrium
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 19 2010

I have just received the following information from the laboratory that tested ab23894: "The secondary we used was a home-made goat anti mouse 1:500 in HRPO stabilizer. It is not commercially available. We do not have a specific protocol. Maybe if yo...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-paraffin sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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