Anti-CD90 / Thy1抗体[5E10] (ab123511)

概述

  • 产品名称Anti-CD90 / Thy1抗体[5E10]
    参阅全部 CD90 / Thy1 一抗
  • 描述
    小鼠单克隆抗体[5E10] to CD90 / Thy1
  • 经测试应用适用于: WB, IP, IHC-Fr, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Dog, Human, Pig, Non Human Primates
  • 免疫原

    HEL erythroleukemia cells.

性能

应用

Our Abpromise guarantee covers the use of ab123511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

Anti-CD90 / Thy1 antibody [5E10] 图像

  • Overlay histogram showing Jurkat cells stained with ab123511 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab123511, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD90 / Thy1 antibody [5E10] (ab123511)参考文献

ab123511 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step Other
Sample Pig Tissue sections (Synovium (hip, knee))
Specification Synovium (hip, knee)
Permeabilization No
Fixative Formaldehyde
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Verified customer

提交于 Aug 30 2013

Thank you for contacting us.

The following antibodies have been tested in WB and human samples, and fulfill most or all of your criteria.

ab123511: 100 ug, conc = 1 mg/ml, but raised against HEL erythroleukemia cells
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"