Anti-CD90 / Thy1抗体[5E10] (ab123511)
Key features and details
- Mouse monoclonal [5E10] to CD90 / Thy1
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD90 / Thy1抗体[5E10]
参阅全部 CD90 / Thy1 一抗 -
描述
小鼠单克隆抗体[5E10] to CD90 / Thy1 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD90/ Thy1. HEL erythroleukemia cells.
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阳性对照
- Flow Cyt: Jurkat cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab123511 was determined to be > 95% pure by SDS-PAGE. -
克隆
单克隆 -
克隆编号
5E10 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)
- PerCP/Cy5.5® Anti-CD90 / Thy1 antibody [5E10] (ab134360)
- Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)
- APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
- PE/Cy5® Anti-CD90 / Thy1 antibody [5E10] (ab95698)
- PE Anti-CD90 / Thy1 antibody [5E10] (ab95700)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123511于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 1 - 3 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use a concentration of 1 - 3 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7070 Human
- Omim: 188230 Human
- SwissProt: P04216 Human
- Unigene: 644697 Human
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别名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
图片
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Overlay histogram showing Jurkat cells stained with ab123511 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab123511, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab123511 被引用在 3 文献中.
- Li X LncRNA metastasis-associated lung adenocarcinoma transcript-1 promotes osteogenic differentiation of bone marrow stem cells and inhibits osteoclastic differentiation of Mø in osteoporosis via the miR-124-3p/IGF2BP1/Wnt/β-catenin axis. J Tissue Eng Regen Med 16:311-329 (2022). PubMed: 34962086
- Jia YY et al. Effect of Optimized Concentrations of Basic Fibroblast Growth Factor and Epidermal Growth Factor on Proliferation of Fibroblasts and Expression of Collagen: Related to Pelvic Floor Tissue Regeneration. Chin Med J (Engl) 131:2089-2096 (2018). PubMed: 30127219
- Liu XL et al. Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural-like cells. Mol Med Rep 17:7902-7910 (2018). PubMed: 29620288