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RabMAb

Anti-CD9抗体[EPR2949] (ab92726)

Abreviews (5)Q&A (7)Specific References (22)

概述

  • 产品名称
    Anti-CD9抗体[EPR2949]
    参阅全部 CD9 一抗
  • 描述
    兔单克隆抗体[EPR2949] to CD9
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human CD9 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P21926

  • 阳性对照
    • WB: HeLa, HuT-78 and U87-MG cell lysates IHC-P: Human breast carcinoma tissue, human endometrial carcinoma and human tonsil tissue
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab92726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 25 kDa.
IP 1/10 - 1/100.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

靶标

  • 功能
    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • 组织特异性
    Expressed by a variety of hematopoietic and epithelial cells.
  • 序列相似性
    Belongs to the tetraspanin (TM4SF) family.
  • 翻译后修饰
    Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Tetraspanin 29 antibody
    • 5H9 antibody
    • 5H9 antigen antibody
    • Antigen defined by monoclonal antibody 602 29 antibody
    • Antigen defined by monoclonal antibody 60229 antibody
    • BA-2/p24 antigen antibody
    • BA2 antibody
    • BTCC 1 antibody
    • BTCC1 antibody
    • CD9 antibody
    • CD9 antigen antibody
    • CD9 antigen p24 antibody
    • CD9 molecule antibody
    • CD9_HUMAN antibody
    • Cell growth inhibiting gene 2 protein antibody
    • Cell growth-inhibiting gene 2 protein antibody
    • DRAP 27 antibody
    • DRAP27 antibody
    • GIG2 antibody
    • Growth inhibiting gene 2 protein antibody
    • Leukocyte antigen MIC3 antibody
    • MIC3 antibody
    • Motility related protein antibody
    • Motility-related protein antibody
    • MRP 1 antibody
    • MRP-1 antibody
    • MRP1 antibody
    • p24 antibody
    • p24 antigen antibody
    • Tetraspanin-29 antibody
    • Tspan 29 antibody
    • Tspan-29 antibody
    • TSPAN29 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

    Unpurified ab92726 showing positive staining in Papillary carcinoma of thyroid gland tissue.

  • Western blot - Anti-CD9 antibody [EPR2949] (ab92726)
    Western blot - Anti-CD9 antibody [EPR2949] (ab92726)
    All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/10000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HuT-78 cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 24 kDa (why is the actual band size different from the predicted?)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemical staining of paraffin embedded rat spleen with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunoprecipitation - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunoprecipitation - Anti-CD9 antibody [EPR2949] (ab92726)

    ab92726 (purified) at 1/20 immunoprecipitating CD9 in 10 μg HeLa (Lanes 1 and 2, observed at 24 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST

    Dilution buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-CD9 antibody [EPR2949] (ab92726)
    Western blot - Anti-CD9 antibody [EPR2949] (ab92726)
    All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/2000 dilution (purified)

    Lane 1 : mouse heart lysate
    Lane 2 : mouse kidney lysate
    Lane 3 : rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 24 kDa (why is the actual band size different from the predicted?)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemical staining of paraffin embedded human tonsil with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

    Unpurified ab92726 showing positive staining in Normal kidney tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

    Unpurified ab92726 showing positive staining in Astrocytoma tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

    Unpurified ab92726 showing positive staining in Normal brain tissue.

文献

This product has been referenced in:
  • Fang T  et al. Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer. Nat Commun 9:191 (2018). Read more (PubMed: 29335551) »
  • Gao Y & Ge W The histone methyltransferase DOT1L inhibits osteoclastogenesis and protects against osteoporosis. Cell Death Dis 9:33 (2018). Read more (PubMed: 29348610) »

See all 22 Publications for this product

发表研究结果有使用 ab92726？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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12 reviews or Q&A

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Votes: Highest First

Western blot abreview for Anti-CD9 antibody [EPR2949]

 Good
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Hep G2)
Specification
Hep G2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jan 05 2015

Western blot abreview for Anti-CD9 antibody [EPR2949]

 Good
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Nov 07 2014

Flow Cytometry abreview for Anti-CD9 antibody [EPR2949]

 Good
Abreviews
Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 70% methanol
Sample
Human Cell (HepG2)
Specification
HepG2
Gating Strategy
Live cells
Preparation
Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
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Verified customer

提交于 Jun 20 2014

Western blot abreview for Anti-CD9 antibody [EPR2949]

 Excellent
Abreviews
Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (HepG2)
Specification
HepG2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Apr 04 2014

I just want to know that your product Anti-CD9 antibody [EPR2949] (ab92726) whether can be used as marker of urinary exosomes in case of Western bolting or not.

We have not specifically tested this Anti-CD9 antibody [EPR2949] (ab92726) as a marker of urinary exosomes but I can confirm that it can be used in western blot on mouse, rat, and human samples. Additionally, I have done a Google search and found that ...

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Hello, I am writing in reference to an anti-CD9 antibody with the Clone ID: EPR2949, and catalog number 2739-1. In the product information, it states that the antibody is cross-reactive with mouse. However, it states that the antibody is recommended ...

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Thank you for contacting us.
Yes, this antibody should work in mouse for Immunofluorescence. The reference below (also on our datasheet) has used this antibody for IF in mouse.

Huang W et al. CD9 tetraspanin interacts with CD36 on the surf...

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Dear Sir/Madam,



I recently purchased anti-CD9 [EPR2949] cat# ab92726. Could you please let me know whether if binds to extracellular or intracellular domain of CD9?

Thanks!

Thank you for your inquiry.

I can confirm that the immunogen of the antibody ab92726 (Anti-CD9 antibody [EPR2949]) is located in the cytoplasmic part of the protein.

This antibody is tested and guaranteed for WB, IP, IHC-P, ICC and ...

Read More

... sind wir auf der Suche nach einem Antikörper, welcher Strukturen anfärbt, die in jedem Gewebe nachweisbar sind. Zu diesem Zweck haben wir uns den Anti-CD9 Antikörper (ab92726) ausgesucht. Wir würden diesen gerne zunächstauf...

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Vielen Dank für Ihre Anfrage.

Leider haben wir keine Teströhrchen von unseren Produkten die wir versenden könnten. Wir garantieren alle Antikörper so zu funktionieren, wie wir sie beschreiben. Das heisst, fallssie denAnti...

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Western blot abreview for Anti-CD9 antibody [EPR2949]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Serum (Purified microvesicles)
Loading amount
10 µg
Specification
Purified microvesicles
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Mar 12 2012

We ordered ab92726 (CD9), ab41803 (Anxa2), ab83 (Tsg101) from Abcam.

What conditions do you recommend for each one of these Abs for running Westerns – reducing or non-reducing?

Thank you for contacting Abcam.

Forthese antibodies I would recommend using reducing conditions and also initially using a 1/1000 primary antibody dilution, although you may to alter this concentration for subsequent western blots.

...

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