- 数据表
- 具体的参考文献 (7)
- 实验方案
概述
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产品名称Anti-CD9抗体[EPR2949]
参阅全部 CD9 一抗 -
描述兔单克隆抗体[EPR2949] to CD9
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经测试应用适用于: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
A synthetic peptide corresponding to residues within Human CD9.
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阳性对照
- WB: HeLa, HuT-78 and U87-MG cell lysates IHC-P: Human breast carcinoma tissue, human endometrial carcinoma and human tonsil tissue
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常规说明
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EPR2949
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同种型IgG
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研究领域
相关产品
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Alternative Versions
- Anti-CD9 antibody [EPR2949] (Alexa Fluor® 488) (ab195348)
- Anti-CD9 antibody [EPR2949] (Alexa Fluor® 647) (ab195349)
- Anti-CD9 antibody [EPR2949] - BSA and Azide free (ab195422)
- Anti-CD9 antibody [EPR2949] (HRP) (ab195552)
- Anti-CD9 antibody [EPR2949] (PerCP) (ab220512)
- Anti-CD9 antibody [EPR2949] (Allophycocyanin) (ab221262)
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab92726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/500. | |
| WB | 1/2000. Predicted molecular weight: 25 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol). |
靶标
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功能Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
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组织特异性Expressed by a variety of hematopoietic and epithelial cells.
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序列相似性Belongs to the tetraspanin (TM4SF) family.
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翻译后修饰Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 928 Human
- Entrez Gene: 12527 Mouse
- Entrez Gene: 24936 Rat
- Omim: 143030 Human
- SwissProt: P21926 Human
- SwissProt: P40240 Mouse
- SwissProt: P40241 Rat
- Unigene: 114286 Human
see all -
别名
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Anti-CD9 antibody [EPR2949] 图像
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![Western blot - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-254274-ab92726WB1.jpg)
Western blot - Anti-CD9 antibody [EPR2949] (ab92726)All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/10000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size : 25 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-16-ab92726ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD9 antibody [EPR2949] (ab92726)Immunofluorescence staining of SW480 cells with purified ab92726 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92726 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-15-ab92726IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)Immunohistochemical staining of paraffin embedded rat spleen with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunoprecipitation - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-18-ab92726IP1.jpg)
Immunoprecipitation - Anti-CD9 antibody [EPR2949] (ab92726)ab92726 (purified) at 1/20 immunoprecipitating CD9 in 10 μg HeLa (Lanes 1 and 2, observed at 24 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: 5% NFDM/TBST
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![Flow Cytometry - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-17-ab92726FC1.jpg)
Flow Cytometry - Anti-CD9 antibody [EPR2949] (ab92726)Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab92726 at a dilution of 1 in 20 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). -
![Western blot - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-254277-ab92726WB2.jpg)
Western blot - Anti-CD9 antibody [EPR2949] (ab92726)All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/2000 dilution (purified)
Lane 1 : mouse heart lysate
Lane 2 : mouse kidney lysate
Lane 3 : rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size : 25 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-14-ab92726IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)Immunohistochemical staining of paraffin embedded human tonsil with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Flow Cytometry - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/ab92726-13-ab92726FC.jpg)
Flow Cytometry - Anti-CD9 antibody [EPR2949] (ab92726)Overlay histogram showing Jurkat cells stained with unpurified ab92726 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92726, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/CD9-Primary-antibodies-ab92726-20.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)Unpurified ab92726 showing positive staining in Papillary carcinoma of thyroid gland tissue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/CD9-Primary-antibodies-ab92726-21.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)Unpurified ab92726 showing positive staining in Normal kidney tissue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/CD9-Primary-antibodies-ab92726-22.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)Unpurified ab92726 showing positive staining in Astrocytoma tissue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)](http://www.abcam.cn/ps/products/92/ab92726/Images/CD9-Primary-antibodies-ab92726-27.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)Unpurified ab92726 showing positive staining in Normal brain tissue.
实验方案
Anti-CD9 antibody [EPR2949] (ab92726)参考文献
This product has been referenced in:
- Yim N et al. Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module. Nat Commun 7:12277 (2016). Electron Microscopy ; Human . Read more (PubMed: 27447450) »
- Sun Y et al. A novel TP53 pathway influences the HGS-mediated exosome formation in colorectal cancer. Sci Rep 6:28083 (2016). WB ; Human . Read more (PubMed: 27312428) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"