Synthetic peptide within Human CD9 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P21926
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab92726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000. Predicted molecular weight: 25 kDa. | |
IP | 1/10 - 1/100. | |
IHC-P | 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Unpurified ab92726 showing positive staining in Papillary carcinoma of thyroid gland tissue.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ab92726 (purified) at 1/20 immunoprecipitating CD9 in 10 μg HeLa (Lanes 1 and 2, observed at 24 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: 5% NFDM/TBST
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Unpurified ab92726 showing positive staining in Normal kidney tissue.
Unpurified ab92726 showing positive staining in Astrocytoma tissue.
Unpurified ab92726 showing positive staining in Normal brain tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"