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Tissue/ cell preparation (Mouse): LPS-activated B cells.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab24862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Flow Cytometry analysis of C57Bl/6 splenocytes unstimulated (left panel) or stimulated for 3 days with LPS (right panel) labeling CD86 with ab24862 at 0.125µg (solid line). Rat IgG2a (FITC) isotype control 0.125µg (dotted line).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"