Anti-CD82抗体[TS82b] (ab59509)

概述

  • 产品名称
    Anti-CD82抗体[TS82b]
    参阅全部 CD82 一抗
  • 描述
    小鼠单克隆抗体[TS82b] to CD82
  • 经测试应用
    适用于: IHC-P, WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Jurkat and HEL cell lines.

  • 阳性对照
    • HEL cells

性能

应用

Our Abpromise guarantee covers the use of ab59509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 37 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
  • 组织特异性
    Lymphoid specific.
  • 序列相似性
    Belongs to the tetraspanin (TM4SF) family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4F9 antibody
    • Antigen detected by monoclonal and antibody IA4 antibody
    • C33 antibody
    • C33 antigen antibody
    • CD 82 antibody
    • CD82 antibody
    • CD82 antigen antibody
    • CD82 molecule antibody
    • CD82_HUMAN antibody
    • GR 15 antibody
    • GR15 antibody
    • IA 4 antibody
    • IA4 antibody
    • Inducible membrane protein antibody
    • Inducible membrane protein R2 antibody
    • KAI 1 antibody
    • KAI1 antibody
    • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
    • Kangai 1 antibody
    • Kangai1 antibody
    • Leukocyte surface antigen R2 antibody
    • Metastasis suppressor Kangai 1 antibody
    • Metastasis suppressor Kangai-1 antibody
    • Metastasis suppressor Kangai1 antibody
    • Prostate cancer antimetastasis gene KAI1 antibody
    • R2 antibody
    • R2 leukocyte antigen antibody
    • SAR 2 antibody
    • SAR2 antibody
    • ST 6 antibody
    • ST6 antibody
    • Suppression of tumorigenicity 6 antibody
    • Suppression of tumorigenicity 6 prostate antibody
    • Suppressor of tumorigenicity 6 antibody
    • Suppressor of tumorigenicity 6 protein antibody
    • Tetraspanin 27 antibody
    • Tetraspanin-27 antibody
    • Tetraspanin27 antibody
    • Tspan 27 antibody
    • Tspan-27 antibody
    • Tspan27 antibody
    see all

图片

  • ab59509 staining CD82 in Rat placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilsed with 0.3% Triton X-100 and blocked with 3% serum for 60 minutes at 23°C; antigen retrieval was by heat mediation in 10mM citrate buffer pH6.0. Samples were incubated with primary antibody (1/200 in blocking buffer) for 12 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ab59509 staining CD82 (left) in Human peripheral blood neutrophils by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% Triton-X 100 + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Middle - LFA 1a, Right - merge.

    See Abreview



  • Predicted band size : 37 kDa
    1.3X105 HEL cells were lysed by the following lysis buffer: 10mM Tris, pH 7.4, 150mM NaCl, 1mM CaCl2, 1mM MgCl2, 0.02% NaN3, 1mM PMSF, 0.5 lg/ml leupeptin, 1 lg/ml pepstatin A, and 10 KIU/ml aprotinin containing 1% detergent (Brij 97 or Triton). The lysate was separated by SDS/PAGE (5–15% gel) under non-reducing conditions and transferred to a PVDF membrane. ab59509 incubation time was 1h. 1,3 105 HEL cells were lysed (lysis buffer containing 1%Brij97 or triton) Migration SDS/page 5-15% gel under non-reducing conditions.
  • Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献

This product has been referenced in:
  • Ni YH  et al. Distinct expression patterns of Toll-like receptor 7 in tumour cells and fibroblast-like cells in oral squamous cell carcinoma. Histopathology 67:730-9 (2015). IHC . Read more (PubMed: 25828894) »
  • Spring FA  et al. Tetraspanins CD81 and CD82 facilitate a4ß1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One 8:e62654 (2013). IP ; Human . Read more (PubMed: 23704882) »

See all 4 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (10 mM citrate sodium, 10 mM citric acid, pH 6.0)
Sample
Rat Tissue sections (Rat placenta tissue)
Specification
Rat placenta tissue
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 03 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Mouse Cell (Mouse erythroblasts)
Specification
Mouse erythroblasts
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 23 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Human Cultured Cells (Human trophoblast cell line)
Specification
Human trophoblast cell line
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 23 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Human Cell lysate - whole cell (Human peripheral blood neutrophils)
Specification
Human peripheral blood neutrophils
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (Human peripheral blood neutrophils)
Specification
Human peripheral blood neutrophils
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 11 2013

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Leider haben wir aus logistischen Gründen keine Aliquots von unseren Antikörpern, die wir Ihnen senden könnten. Ich kann Ihnen jedoch versichern, dass wir unsere Produkte garantieren so zu ...

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The theoretical weight of CD82 is around 34 kD (in reducing conditions) The band size is very difficult to determine because it migrates as a smear, due to high level of N-Glycosylation. This glycosylation could depend on the cell type and the state of...

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