概述

  • 产品名称Anti-CD80抗体
    参阅全部 CD80 一抗
  • 描述
    兔多克隆抗体to CD80
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse CD80.

    (Peptide available as ab66476.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Mouse Spleen Tissue; Mouse Thymus Tissue; Mouse Brain Tissue; Mouse Spinal Cord Tissue; EL4 Whole Cell; RAW 264.7 Whole Cell

性能

应用

Our Abpromise guarantee covers the use of ab64116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33, 65 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • 组织特异性Expressed on activated B-cells, macrophages and dendritic cells.
  • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activation B7-1 antigen antibody
    • B lymphocyte activation antigen B7 antibody
    • B7 antibody
    • B7-1 antibody
    • B7-1 antigen antibody
    • B7.1 antibody
    • BB1 antibody
    • CD28 antigen ligand 1 antibody
    • CD28LG antibody
    • CD28LG1 antibody
    • CD80 antibody
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
    • CD80 antigen antibody
    • CD80 molecule antibody
    • CD80_HUMAN antibody
    • Costimulatory factor CD80 antibody
    • costimulatory molecule variant IgV-CD80 antibody
    • CTLA-4 counter-receptor B7.1 antibody
    • LAB7 antibody
    • T-lymphocyte activation antigen CD80 antibody
    see all

Anti-CD80 antibody 图像

  • All lanes : Anti-CD80 antibody (ab64116) at 1 µg/ml

    Lane 1 : Spleen (Mouse) Tissue Lysate
    Lane 2 : Thymus (Mouse) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 5 : Spinal Cord (Mouse) Tissue Lysate
    Lane 6 : EL4 whole cell lysate (ab7183)
    Lane 7 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 33,65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    CD80 has a predicted molecular weight of 33 kDa, however it is predicted to be extensively glycosylated (SwissProt data). The band seen at 65 kDa is consistent with the banding pattern observed for other commercially available antibodies to CD80. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • ab64116 (1:40) staining CD80 in paraffin-embedded mouse lymph node tissue (left panel) using an automated system (Ventana Discovery).  Right-hand panel shows negative control (no primary antibody).  
    Using this protocol there is strong membrane staining, of activated B cells in the germinal centres.  What appear to be scattered activated B cells or possibly plasma cells in the interfollicular areas of the lymph node and blood vessels.

    Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab64116 (1:40 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8

Anti-CD80 antibody (ab64116)参考文献

This product has been referenced in:
  • Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). Read more (PubMed: 25845433) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spleen)
Antigen retrieval step None
Permeabilization No
Specification Spleen
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
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提交于 Jan 19 2015

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