概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD80
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse CD80.

    (Peptide available as ab66476.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Mouse Lymph node

性能

应用

Our Abpromise guarantee covers the use of ab64116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • 组织特异性
    Expressed on activated B-cells, macrophages and dendritic cells.
  • 序列相似性
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activation B7-1 antigen antibody
    • B lymphocyte activation antigen B7 antibody
    • B7 antibody
    • B7-1 antibody
    • B7-1 antigen antibody
    • B7.1 antibody
    • BB1 antibody
    • CD28 antigen ligand 1 antibody
    • CD28LG antibody
    • CD28LG1 antibody
    • CD80 antibody
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
    • CD80 antigen antibody
    • CD80 molecule antibody
    • CD80_HUMAN antibody
    • Costimulatory factor CD80 antibody
    • costimulatory molecule variant IgV-CD80 antibody
    • CTLA-4 counter-receptor B7.1 antibody
    • LAB7 antibody
    • T-lymphocyte activation antigen CD80 antibody
    see all

图片

  • ab64116 (1:40) staining CD80 in paraffin-embedded mouse lymph node tissue (left panel) using an automated system (Ventana Discovery).  Right-hand panel shows negative control (no primary antibody).  
    Using this protocol there is strong membrane staining, of activated B cells in the germinal centres.  What appear to be scattered activated B cells or possibly plasma cells in the interfollicular areas of the lymph node and blood vessels.

    Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab64116 (1:40 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8

文献

This product has been referenced in:
  • Xu S  et al. miR-424(322) reverses chemoresistance via T-cell immune response activation by blocking the PD-L1 immune checkpoint. Nat Commun 7:11406 (2016). WB . Read more (PubMed: 27147225) »
  • Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). Read more (PubMed: 25845433) »

See all 2 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
None
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

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Verified customer

提交于 Jan 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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