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Our Abpromise guarantee covers the use of ab24860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|Functional Studies||Use at an assay dependent concentration.
Also suitable for in vivo and in vitro blocking of ligand binding.
|IP||Use at an assay dependent concentration.|
P815 cells transfected with CD80 or non-transfected P815 control cells were stained with ab24860 (3 µg/106 cells). Large cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
ab24860 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"