Anti-CD8抗体[UCHT-4] (ab13442)

概述

  • 产品名称Anti-CD8抗体[UCHT-4]
    参阅全部 CD8 一抗
  • 描述
    小鼠单克隆抗体[UCHT-4] to CD8
  • 特异性Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human thymocytes followed by peripheral blood T cells.

  • 常规说明If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab13442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 5µl for 106 cells.

    Monoclonal Anti-Human CD8 may be used for:
    1. Enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids.
    2. Identification and localization of T cytotoxic/suppressor lymphocytes in lymphoid and other tissues.
    3. Analysis of cell mediated cytotoxicity.
    4. Studies of immunoregulation in health and disease.
    5. Investigation of NK cells.
    6. Complement mediated cytolysis of CD8 expressing cells.

    For fluorescence analysis of cells with Fc receptors, the use of isotype matched negative control is mandatory. In some systems it may be necessary to incubate the cells in 10-20% normal serum from the second antibody host species (at step 2 before adding monoclonal antibody) in order to decrease non-specific staining with
    the conjugated second antibody.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • 疾病相关Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻译后修饰All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 形式CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
    • 别名
      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    Anti-CD8 antibody [UCHT-4] (ab13442)参考文献

    ab13442 has not yet been referenced specifically in any publications.

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